Molecular Cloning and Expression of Ppdc-ldhL Fusion Gene in Zymomonas Mobilis ATCC 10988

In this study, the 5' terminus of ldhL gene in Lactobacillus casei ATCC 334 was fused to the 3' terminus of pdc gene promoter (P pdc ) part in Zymomonas mobilis ATCC 10988 by recombinant PCR. The fusion gene P pdc -ldhL was integrated in broad-host-range plasmid pBBR1MCS3, transformed by e...

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Bibliographic Details
Main Authors: Nian-Fa Gao, Yong Wang, Qiang Gao, Bin-Zhe Wang, Jian Zhang, Bo-Ya Liu
Format: Conference Proceeding
Language:English
Subjects:
Biochemistry
Bioinformatics
Biotechnology
Capacitive sensors
Chemical industry
Cloning
DNA
electroporation
Ethanol
L-lactate dehydrogenase
Lactobacillus casei
Nitrogen
pBBR1MCS3
Production
Zymomonas mobilis
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Summary:In this study, the 5' terminus of ldhL gene in Lactobacillus casei ATCC 334 was fused to the 3' terminus of pdc gene promoter (P pdc ) part in Zymomonas mobilis ATCC 10988 by recombinant PCR. The fusion gene P pdc -ldhL was integrated in broad-host-range plasmid pBBR1MCS3, transformed by electroporation and expressed in Z. mobilis ATCC 10988. The primary expression of heterologous lactate dehydrogenase in Z. mobilis lays the foundation to expand the application range of higher value products by Z. mobilis.
DOI:10.1109/IJCBS.2009.108