Forming self-assembled cell arrays and measuring the oxygen consumption rate of a single live cell

We report a method for forming arrays of live single cells on a chip using polymer micro-traps made of SU8. We have studied the toxicity of the microfabricated structures and the associated environment for two cell lines. We also report a method for measuring the oxygen consumption rate of a single...

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Bibliographic Details
Published in:TRANSDUCERS 2009 - 2009 International Solid-State Sensors, Actuators and Microsystems Conference Actuators and Microsystems Conference, 2009-06, p.2374-2377
Main Authors: Etzkorn, J.R., McQuaide, S.C., Anderson, J.B., Meldrum, D.R., Parviz, B.A.
Format: Article
Language:English
Subjects:
Biomedical optical imaging
Biosensors
Fluid flow
Glass
Microfluidics
Optical sensors
Oxygen
oxygen measurement
Polymers
Self-assembly
Semiconductor device measurement
Single-cell
SU8 toxicity
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Summary:We report a method for forming arrays of live single cells on a chip using polymer micro-traps made of SU8. We have studied the toxicity of the microfabricated structures and the associated environment for two cell lines. We also report a method for measuring the oxygen consumption rate of a single cell using optical interrogation of molecular oxygen sensors placed in micromachined micro-wells by temporarily sealing the cells in the micro-traps. The new techniques presented here add to the collection of tools available for performing ldquosingle-cellrdquo biology. A single-cell self-assembly yield of 61% was achieved with oxygen draw down rates of 0.83, 0.82, and 0.71 fmol/minute on three isolated live A549 cells.
ISSN:2159-547X
DOI:10.1109/SENSOR.2009.5285450