Microcantilever-based label-free thermal characterization of biomolecular affinity binding

We present a microfluidic polymer cantilever array device integrating on-chip temperature control for label-free, temperature-dependent characterization of ligand-protein binding. The microfluidic device features a parylene microcantilever array within a microchamber formed by a PDMS spacer layer an...

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Bibliographic Details
Main Authors: Bin Wang, Fengliang Huang, ThaiHuu Nguyen, Qiao Lin
Format: Conference Proceeding
Language:English
Subjects:
Glass
Indium tin oxide
Kinetic theory
Microfluidics
Polymers
Sensor arrays
Temperature control
Temperature dependence
Temperature sensors
Thermoelectric devices
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Summary:We present a microfluidic polymer cantilever array device integrating on-chip temperature control for label-free, temperature-dependent characterization of ligand-protein binding. The microfluidic device features a parylene microcantilever array within a microchamber formed by a PDMS spacer layer and a glass slide including a patterned ITO temperature sensor. The temperature is accurately controlled by using a Peltier device combined with the in situ ITO temperature sensor following a closed-loop control algorithm. For the first time, we use this cantilever-based microfluidic device to systematically characterize the temperature-dependent binding kinetics of an aptamer sensitive to platelet-derived growth factor (PDGF) at temperatures between 19 and 37 °C. Quantitative binding properties including the association and dissociation rate constants (k on and k off ) and equilibrium dissociation constant (K d ) are derived, which indicate significant dependencies on temperature.
ISSN:1084-6999
DOI:10.1109/MEMSYS.2010.5442341