Cloning, Expression and Immunocompetence Analysis of Gene Encoding the Glutamate Dehydrogenase of Streptococcus suis Serotype 2

This study aimed to study the immune activity of glutamate dehydrogenase (GDH) of streptococcus suis serotype 2 and detect its activity. METHODS: The GDH gene was cloned and sequenced. Recombinant GDH protein was expressed in a prokaryotic expression system and purified by affinity chromatography. I...

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Bibliographic Details
Main Authors: Wei-Jun Yang, Zhi-Biao Yang, Xue-Hui Cai, Yong-Qing Hao, Sheng-Bo Zheng, Dao-Liang Lan, Jiang-Ping Gu, Rui Xing, Cong-Li Yuan, Li Cui, Xiu-Guo Hua
Format: Conference Proceeding
Language:English
Subjects:
Animals
Biomembranes
Capacitive sensors
Cloning
DNA
Encoding
Humans
Immune system
Microorganisms
Proteins
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Summary:This study aimed to study the immune activity of glutamate dehydrogenase (GDH) of streptococcus suis serotype 2 and detect its activity. METHODS: The GDH gene was cloned and sequenced. Recombinant GDH protein was expressed in a prokaryotic expression system and purified by affinity chromatography. Immunocompetenc of the resulting protein was analyzed by western blotting with anti-GDH monoclonal antibody. RESULTS: The fusion protein was efficiently expressed. Western blotting analysis confirmed that the resulting protein was immunogenic. CONCLUSIONS: The GDH gene of S.suis 2 was successfully cloned and the high expressed, which facilitated the further studies on the development of immunological detection and vaccine design.
ISSN:2151-7614
2151-7622
DOI:10.1109/ICBBE.2010.5515736