Regulatory network of microRNAs in RAW 264.7 macrophage cells

microRNAs (miRNA) play important roles in regulating immunity. Here we utilize the systems biology approach to predict the regulatory network of miRNAs among the most down-regulated genes by the lipopolysaccharides (LPS) treatment in the macrophage RAW267.4 cell line. We combine the proteome and tra...

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Bibliographic Details
Published in:2010 Annual International Conference of the IEEE Engineering in Medicine and Biology 2010-01, p.6198-6201
Main Authors: Garmire, L X, ZhouXin Shen, Briggs, S, Gene Yeo, Subramaniam, S, Glass, C
Format: Article
Language:English
Subjects:
Bioinformatics
Diseases
Genomics
Immune system
Probes
Proteins
RNA
Online Access:Request full text
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Summary:microRNAs (miRNA) play important roles in regulating immunity. Here we utilize the systems biology approach to predict the regulatory network of miRNAs among the most down-regulated genes by the lipopolysaccharides (LPS) treatment in the macrophage RAW267.4 cell line. We combine the proteome and transcriptome data sets to define 200 target genes that are significantly down-regulated by the LPS treatment. We perform the profiling of over 300 miRNAs with the RNA-Seq method. Using the complementary binding rule between the seed sequences of profiled miRNAs and the 3'UTRs of target genes, we predict genes involved in mobility, metabolism, and oxidative phosphorylation as the top targets of miRNA negative regulation.
ISSN:1094-687X
1558-4615
DOI:10.1109/IEMBS.2010.5627742