Development of a spectrofluorometer with USB interface for in vivo measurements in surgical procedures

In this paper we present the development of a portable spectrofluorometer with USB interface for in vivo measurements in biological tissue. The equipment consists of a mini-spectrometer HR4000CG-UV-NIR, with a working range from 200 to 1100 nm with a resolution of 0.5 nm FWHM. As the excitation sour...

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Bibliographic Details
Main Authors: Fabila, D A, de la Rosa, J M, Stolik, S, Moreno, E, López, T, Álvarez, M, Arellano, A, de la Rosa, G, Mercado, R, Soto, J L
Format: Conference Proceeding
Language:English
Subjects:
Fluorescence
In vivo
Optical fiber amplifiers
Reflection
Spectroscopy
Tumors
Online Access:Request full text
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Summary:In this paper we present the development of a portable spectrofluorometer with USB interface for in vivo measurements in biological tissue. The equipment consists of a mini-spectrometer HR4000CG-UV-NIR, with a working range from 200 to 1100 nm with a resolution of 0.5 nm FWHM. As the excitation source a light emitting diode at 365 nm, with maximum radiant power of 200 mW was used. A bifurcated fiber optic (R200-UV/VIS) with six 200 μm diameter irradiation fibers and one 200 μm collection fiber is used. The system is powered and controlled with a laptop, avoiding the use of an external power supply. A program to select the light source, to process and measure spectra in real time was developed using the graphic language G of LabVIEW 8.2. Also, in this issue are presented in vivo measurements of diffuse reflection and fluorescence of non-neoplastic and neoplastic cerebral tissue of patients affected with glioblastoma multiform tumors (GBM).
DOI:10.1109/CASME.2010.5706669