Advanced EWOD-based digital microfluidic system for multiplexed analysis of biomolecular interactions

This paper presents a low-cost technique for the fabrication of complex electrowetting-on-dielectric (EWOD) digital microfluidic devices. Using this original technology, we have developed devices in which 560 electrodes are used to mix and split nl-size liquid droplets and transport them to 100 anal...

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Bibliographic Details
Main Authors: Brassard, D, Malic, L, Miville-Godin, C, Normandin, F, Veres, T
Format: Conference Proceeding
Language:English
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Summary:This paper presents a low-cost technique for the fabrication of complex electrowetting-on-dielectric (EWOD) digital microfluidic devices. Using this original technology, we have developed devices in which 560 electrodes are used to mix and split nl-size liquid droplets and transport them to 100 analysis spots patterned on a disposable plastic top plate. We demonstrate the multiplexing capability of the developed devices by creating on-chip arrays of droplets with various concentration gradients. Finally, automated biomolecular immobilization and hybridization assays are performed in nl-size droplets under numerous conditions simultaneously with only a limited number of stock solutions.
ISSN:1084-6999
DOI:10.1109/MEMSYS.2011.5734384