Advanced EWOD-based digital microfluidic system for multiplexed analysis of biomolecular interactions
This paper presents a low-cost technique for the fabrication of complex electrowetting-on-dielectric (EWOD) digital microfluidic devices. Using this original technology, we have developed devices in which 560 electrodes are used to mix and split nl-size liquid droplets and transport them to 100 anal...
Saved in:
Main Authors: | , , , , |
---|---|
Format: | Conference Proceeding |
Language: | English |
Subjects: | |
Online Access: | Request full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | This paper presents a low-cost technique for the fabrication of complex electrowetting-on-dielectric (EWOD) digital microfluidic devices. Using this original technology, we have developed devices in which 560 electrodes are used to mix and split nl-size liquid droplets and transport them to 100 analysis spots patterned on a disposable plastic top plate. We demonstrate the multiplexing capability of the developed devices by creating on-chip arrays of droplets with various concentration gradients. Finally, automated biomolecular immobilization and hybridization assays are performed in nl-size droplets under numerous conditions simultaneously with only a limited number of stock solutions. |
---|---|
ISSN: | 1084-6999 |
DOI: | 10.1109/MEMSYS.2011.5734384 |