Reprogramming of cells using modified mRNA

Reprogramming of cells for delivery of proteins at specific sites holds great promise for future clinical applications. Chitosan delivery agents in form of nano-spheres presents a readily available biodegradable option for controlled release of mRNA in the cytosol of the cells. In this work we are u...

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Bibliographic Details
Main Authors: Pandit, V, Nesbitt, R S, Macione, J, Kotha, S P
Format: Conference Proceeding
Language:English
Subjects:
Biomedical imaging
Fluorescence
Immune system
Media
Polymers
Proteins
Stem cells
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Summary:Reprogramming of cells for delivery of proteins at specific sites holds great promise for future clinical applications. Chitosan delivery agents in form of nano-spheres presents a readily available biodegradable option for controlled release of mRNA in the cytosol of the cells. In this work we are using Enhanced Green Fluorescent protein as a proof of concept to show for transfection of osteoblast cells with modified mRNA. Transfection was carried out using lipofectaime RNAiMAX.
ISSN:2160-6986
2160-7028
DOI:10.1109/NEBC.2011.5778580