Label-free live brain imaging with third-harmonic generation microscopy

We demonstrate that optical third-harmonic generation (THG) microscopy enables high-resolution live brain imaging without the need for external contrast agents. We find that the specific geometry and lipid content of brain tissue leads to partial phase-matching of THG outside the neuronal cell bodie...

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Bibliographic Details
Main Authors: Witte, S., Negrean, A., Lodder, J. C., Silva, G. T., de Kock, C. P. J., Mansvelder, H. D., Groot, M. L.
Format: Conference Proceeding
Language:English
Subjects:
Biomedical optical imaging
Brain
Fiber optics
Microscopy
Neurons
Optical harmonic generation
Online Access:Request full text
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Summary:We demonstrate that optical third-harmonic generation (THG) microscopy enables high-resolution live brain imaging without the need for external contrast agents. We find that the specific geometry and lipid content of brain tissue leads to partial phase-matching of THG outside the neuronal cell bodies, producing high-contrast shadow images in which neurons are clearly visible.
DOI:10.1109/CLEOE.2011.5943285