A test target for estimating the resolution of a holographic microscope

A method for testing the resolution of a holographic microscope with a set of in-house test targets is presented. The test targets are made of calibrated microparticles mixed in clear polyurethane medium and sealed between two glass plates. The depth range inside the target that can be imaged with a...

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Bibliographic Details
Main Authors: Kaikkonen, V. A., Makynen, A. J.
Format: Conference Proceeding
Language:English
Subjects:
Detectors
diffraction
Image reconstruction
Image resolution
Laser
Light sources
Microscopy
Pixel
resolving power
Signal to noise ratio
Online Access:Request full text
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Description
Summary:A method for testing the resolution of a holographic microscope with a set of in-house test targets is presented. The test targets are made of calibrated microparticles mixed in clear polyurethane medium and sealed between two glass plates. The depth range inside the target that can be imaged with adequate lateral resolution with different holographic microscope setups is demonstrated by studying resolvability of the microparticles from the reconstructed hologram images using Sparrow's definition for the resolution limit. In addition, the effect of different particle concentrations to image signal-to-noise-ratio is evaluated.
ISSN:1091-5281
DOI:10.1109/IMTC.2011.5944285