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Optimization of ultrasound-assisted extraction of malania oleifera chum polysaccharides using response surface methodology and its antioxidative effect

Response surface methodology (RSM) was used for optimizing ultrasound-assisted extraction of malania polysaccharides. Based on single-factor experiments, three independent variables, namely extraction time(A), ultrasonic temperature(B), and material/water ratio(C), were selected as casual factors. T...

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Bibliographic Details
Main Authors: Tingfan Tang, Xiongmin Liu
Format: Conference Proceeding
Language:chi ; eng
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Summary:Response surface methodology (RSM) was used for optimizing ultrasound-assisted extraction of malania polysaccharides. Based on single-factor experiments, three independent variables, namely extraction time(A), ultrasonic temperature(B), and material/water ratio(C), were selected as casual factors. The experiments were arranged according to Box-Behnken central composite experiment design. Response surface methodology was applied to determine the effect of central composite experiment design and casual factors on the yield of malania polysaccharides(F) which were determined by the phenol-sulfuric acid method. In this foundation, Design Expert software was employed and a second order quadratic equation for malania polysaccharides extraction was established: F=4.29+0.16A+0.18B+0.39C-0.080AB+0.072AC+0.23JBC-0.45A 2 +0.21B 2 -0.15C 2 . It showed that the factors of affecting the extraction rate of malania polysaccharides in order were as follows: material/water ratio(C) >; ultrasonic temperature(B) >; extraction time(A). The model equation was very significant (P
DOI:10.1109/RSETE.2011.5965965