Preliminary study of PASP-USPIO in normal rabbit MR brain perfusion

• Main Authors: Huimao Zhang, Kun Liu, Qiong Yu, Haishan Yang, Xusheng Mao
• Format: Conference Proceeding
• Language: English
• Subjects: Blood; Blood flow; Iron; Magnetic resonance imaging; MRI; perfusion-weighted imaging; poly-aspartic acid; Rabbits; USPIO
• DOI: 10.1109/HHBE.2011.6028034

Objective To explore the practicability and optimal dosage of polyaspartic acid (PASP)-surface-modified ultra-small superparamagnetic iron oxide (USPIO) (PASP-USPIO) in normal rabbit brain perfusion. Methods Thirty New Zealand rabbits were randomly divided into groups A1, A2, A3, A4 and C. Animals in groups A1-4 were intravenously injected (i.v.) with 5 μmol/kg, 10 μmol/kg, 20 μmol/kg and 40 μmol/kg PASP-USPIO, while animals in group C were injected (i.v.) with 0.2 mmol/kg gadolinium diethyl triamine-pentoacetic acid (Gd-DTPA), serving as a control group. Each group was subjected to T 2 * Gradient Echo-Echo Planar Imaging (T 2 * GRE-EPI)-sequenced perfusion-weighted imaging to obtain signal intensity-time curves, which were used to calculate the regional cerebral blood volume (rCBV), the maximum signal drop percentage (SRR max ), and Q rCBV -Q SRRma X values. Data were compared within and between groups. Results The intra-group rCBV value and SRR max value were significantly different between gray matter and white matter. The inter-group Q rCBV and Q SRRmax values were also significantly different. ANOVA analysis indicated that the Q rCBV and Q SRRmax values in group A4 were significantly different from those in other groups, while there were no significant differences among group A1, A2, A3 and C. Conclusion PASP-USPIO has colloid stability and can be used in normal rabbit MR brain perfusion. The optimal dosage is 10 μmol/kg.