3D biomimetic chip integrated with microvascular system for studying the liver specific functions

We describe the design and fabrication of 3D chip that enables the cell co-culture under continuous flow perfusion and repeated in situ observation by light microscopy. Thin polydimethylsiloxane (PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels...

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Bibliographic Details
Main Authors: Kuo-Wei Chang, Chia-Tung Lee, Harishchandra, P. T., Hung-Po Chen, Ting-Ru Yueh, Valagerahally Puttaswamy, Srinivasu, Sivashankar, S., Cheng-Hsien Liu
Format: Conference Proceeding
Language:English
Subjects:
3D chip
Arrays
Blood
Lasers
Liver
mimetic liver
Nanoelectromechanical systems
Semiconductor device modeling
Thin PDMS membrane
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Summary:We describe the design and fabrication of 3D chip that enables the cell co-culture under continuous flow perfusion and repeated in situ observation by light microscopy. Thin polydimethylsiloxane (PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels to form an upper chamber and lower chamber. In the upper chamber hepatocytes (HepG2) and fibroblasts (3T3) were co-cultured to mimic the liver whereas in the lower structure endothelial cells (HMEC-1) were cultured to mimic the vascular system at the cellular scale for providing nutrients to the in vitro liver mimicked in the upper channel.
DOI:10.1109/NEMS.2012.6196760