Layer-by-layer 3-D constructs of adherent and non-adherent cells in hydrogel

We report a protocol for the layer-by-layer fabrication of adherent and non-adherent 3-dimensional (3-D) cell cultures. Adherent Madin Darby canine kidney (MDCK) epithelial cell cultures were patterned onto Petri dishes, and non-adherent cell cultures were physically encapsulated in alginate hydroge...

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Bibliographic Details
Main Authors: Phamduy, T. B., Raof, N. A., Xie, Y., Chrisey, D. B.
Format: Conference Proceeding
Language:English
Subjects:
Arrays
Encapsulation
Fabrication
Lasers
Media
Protocols
Substrates
Online Access:Request full text
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Summary:We report a protocol for the layer-by-layer fabrication of adherent and non-adherent 3-dimensional (3-D) cell cultures. Adherent Madin Darby canine kidney (MDCK) epithelial cell cultures were patterned onto Petri dishes, and non-adherent cell cultures were physically encapsulated in alginate hydrogel layer above the adherent cells. The layer-by-layer, CAD/CAM fabrication process results in soft transfer and does not harm adherent cells. We have developed a technique to fabricate a multi-layer cell culture system to study the interactions between adherent and non-adherent cell types.
ISSN:2160-6986
2160-7028
DOI:10.1109/NEBC.2012.6207078