Lifetime estimation of moving vesicles in frequency-domain fluorescence lifetime imaging microscopy

We propose a robust statistical framework for correcting the movements of vesicles in frequency domain FLIM imaging. Movement and lifetime are jointly estimated in a three-step procedure. Robust M-estimators are mainly used to improve accuracy in temporally-varying noisy images. The performance of t...

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Bibliographic Details
Main Authors: Roudot, P., Kervrann, C., Waharte, F.
Format: Conference Proceeding
Language:English
Subjects:
Estimation
FLIM
Fluorescence
Frequency domain analysis
frequency-domain
M-estimation
Microscopy
phase modulation
Robustness
spot tracking
template matching
Tracking
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Description
Summary:We propose a robust statistical framework for correcting the movements of vesicles in frequency domain FLIM imaging. Movement and lifetime are jointly estimated in a three-step procedure. Robust M-estimators are mainly used to improve accuracy in temporally-varying noisy images. The performance of the proposed method is demonstrated on both simulated and real samples.
ISSN:1945-7928
1945-8452
DOI:10.1109/ISBI.2012.6235636