Optimal 3D single-molecule super-resolution microscopy with engineered point spread functions

Three-dimensional single-molecule localization is a fundamental problem in biological and biophysical experiments such as super-resolution microscopy. Point spread function engineering accompanied by corresponding reconstruction procedures provides a unique approach to increase localization precisio...

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Bibliographic Details
Main Authors: Quirin, S., Grover, G., Piestun, R.
Format: Conference Proceeding
Language:English
Subjects:
Fluorescence
Image resolution
Microscopy
Optical microscopy
Photonics
Point spread function engineering
Single-molecule localization
Superresolution microscopy
USA Councils
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Description
Summary:Three-dimensional single-molecule localization is a fundamental problem in biological and biophysical experiments such as super-resolution microscopy. Point spread function engineering accompanied by corresponding reconstruction procedures provides a unique approach to increase localization precision. Joint optical/digital design and image reconstruction are investigated.
ISSN:1945-7928
1945-8452
DOI:10.1109/ISBI.2012.6235707