Comparison of Inhibitory Effects of Nine Flavonoids on Prostaglandin E2 Production and COX-2 Expression in LPS-Stimulated RAW264.7 Macrophages

Prostaglandin E 2 (PGE2) has a crucial role in inflammation and is synthesized by cyclooxygenase-2 (COX-2), of which the expression is induced in inflammatory site by inflammatory stimuli like lipopolysaccharide (LPS). In the present study, nine flavonoids were tested for their effects on prostaglan...

Full description

Saved in:
Bibliographic Details
Main Authors: Yirong Xu, Changlu Wang, Longyan Wu, Zhenjing Li, Mianhua Chen, Yurong Wang, Fengjuan Li, Cheng Luo
Format: Conference Proceeding
Language:English
Subjects:
Cells (biology)
Compounds
cyclooxygenase-2
Drugs
Educational institutions
flavonoids
Immune system
inflammation
isoquercitrin
Mice
Production
prostaglandin E2
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Prostaglandin E 2 (PGE2) has a crucial role in inflammation and is synthesized by cyclooxygenase-2 (COX-2), of which the expression is induced in inflammatory site by inflammatory stimuli like lipopolysaccharide (LPS). In the present study, nine flavonoids were tested for their effects on prostaglandin E 2 production and cyclooxygenase-2 expression in LPS-stimulated RAW 264.7 macrophages. All the nine compounds, including apigenin, lute Olin, scutellarein, quercetin, myricetin, isoquercitrin, genistein, hyperoside, rutin effectively inhibited the LPS-induced PGE2 production but only isoquercitrin showed the significant inhibitory effect on the COX-2 expression. It was found that isoquercitrin exhibited a concentration-dependent inhibition of PGE2 production and COX-2 expression with the estimated IC50 values of 25 μM and 65 μM, respectively. The results introduce isoquercitrin as a good compound capable of down regulating the expression of COX-2.
DOI:10.1109/iCBEB.2012.108