A novel strategy to tag MMPs-positive cells for in vivo imaging of tumor cells

Matrix metalloproteinases (MMPs) are endopeptidases responsible for degrading the extracellular matrix (ECM) and remodeling tissue in both physiological and pathological processes. MMP2 and membrane-type 1 MMP (MT1-MMP) have been associated with tumor invasion, metastasis and angiogenesis; therefore...

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Bibliographic Details
Main Authors: Tao Zhao, Xia Miao, Lihua Zeng, Haiyang Lang, Shenglong Xu, Dongqing Ren, Yongbin Chen, Yang Liu, Yurong Li, Guozhen Guo
Format: Conference Proceeding
Language:English
Subjects:
ECM
Fluorescence
Humans
Invasion
Metastasis
MMPs
Molecular imaging
Proteins
Tumor malignancy
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Summary:Matrix metalloproteinases (MMPs) are endopeptidases responsible for degrading the extracellular matrix (ECM) and remodeling tissue in both physiological and pathological processes. MMP2 and membrane-type 1 MMP (MT1-MMP) have been associated with tumor invasion, metastasis and angiogenesis; therefore, a molecular imaging strategy assessing their activity may help to predict the malignancy of tumors. Here, we established a novel method of specifically tagging the surface of MMP2- and MT1-MMP-positive cells, and applied it to the development of an optical imaging probe. We constructed a protein-based probe. In vitro and in vivo experiments demonstrated that the probe was cleaved and specifically remained in tumor xenografts in a MMP-dependent manner.
DOI:10.1109/CEEM.2012.6410634