Enzyme-doped ion selective membrane (ED-ISM) formed with surface force and microstrucures for high perforlance urea detection

This research presents a novel technique to form a thin enzyme-doped ion selective membrane (ED-ISM) in a microfluidic chip utilizing surface tension force for on-site bio-reaction, ion selection and electrochemical detection of urea. Diamond shape microstructures assisted with neighboring air sheat...

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Bibliographic Details
Main Authors: Chiang, Ting-Yi, Lin, Che-Hsin
Format: Conference Proceeding
Language:English
Subjects:
Biochemistry
Biomembranes
Devices
Electrodes
Enzymes
Force
Mechanical systems
Membranes
Microfluidics
Polymers
Sheaths
Solvents
Ureas
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Summary:This research presents a novel technique to form a thin enzyme-doped ion selective membrane (ED-ISM) in a microfluidic chip utilizing surface tension force for on-site bio-reaction, ion selection and electrochemical detection of urea. Diamond shape microstructures assisted with neighboring air sheath flows are used to trap trace amount of polymer liquid for ED-ISM formation. Since bio-reactive enzyme of urease is immobilized on the ISM, passing urea molecules are converted into ammonia ion then diffuse through the ISM for further electrochemical detection. The produced ED-ISM has a thinner thickness (
ISSN:1084-6999
DOI:10.1109/MEMSYS.2013.6474460