Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells

Fabrication of 3D chip that enables cell culture under continuous flow perfusion and repeated in situ observation by light microscopy is described. Thin polydimethylsiloxane (PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels to form an upper cham...

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Main Authors: Pei-Yu Chang, Kuo-Wei Chang, Ciou-Wen Lin, Po-Chen Shih, Cheng-Hsien Liu, Chin-Jung Li, Chang-Hung Tien, Hong-Yuan Huang, Wen-Syang Hsu, Da-Jeng Yao, Shih-Kang Fan
Format: Conference Proceeding
Language:English
Subjects:
3D chip
mimetic uterus
Thin membrane
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Summary:Fabrication of 3D chip that enables cell culture under continuous flow perfusion and repeated in situ observation by light microscopy is described. Thin polydimethylsiloxane (PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels to form an upper chamber and lower chamber. Thus we fabricated a 3D microfluidic chip which integrates the concentration gradient design and perfusion system for proposed embryo coculture with stromal cells.
ISSN:2159-6964
DOI:10.1109/NANOMED.2012.6509134