Loading…

Poly(3,4-ethylenedioxythiophene) on decellular scaffolding interrupts grafted muscle revascularization

Regenerative peripheral nerve interfaces (RPNIs) are implantable devices for amputee communication between peripheral nerves and microprocessor controlled robotic prostheses. Our RPNIs consist of transferred muscles reinnervated by the peripheral nerves remaining in the limb post amputation. RPNI co...

Full description

Saved in:
Bibliographic Details
Main Authors: Baldwin, Joseph F., Washabaugh, E. Peter, Moon, Jana D., Shim, Bong-Sup, Martin, David C., Cederna, Paul S., Langhals, Nicholas B., Urbanchek, Melanie G.
Format: Conference Proceeding
Language:English
Subjects:
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 1464
container_issue
container_start_page 1461
container_title
container_volume
creator Baldwin, Joseph F.
Washabaugh, E. Peter
Moon, Jana D.
Shim, Bong-Sup
Martin, David C.
Cederna, Paul S.
Langhals, Nicholas B.
Urbanchek, Melanie G.
description Regenerative peripheral nerve interfaces (RPNIs) are implantable devices for amputee communication between peripheral nerves and microprocessor controlled robotic prostheses. Our RPNIs consist of transferred muscles reinnervated by the peripheral nerves remaining in the limb post amputation. RPNI construction includes a light muscle wrap of decellularized small-intestine submucosa (SIS). We studied RPNI revascularization and regeneration when SIS had an electrically conductive coating of poly 3,4 ethylene dioxytheophene (PEDOT). Rats underwent free-muscle transfer (FMT). The peroneal nerve was divided and the proximal end was implanted in the FMT. Experimental groups received either: a) no SIS (NONE), b) SIS (SIS) wrap, or c) wrap of SIS polymerized with PEDOT, (SIS+PEDOT). One week later, rats were perfused with lead oxide, vascular areas were quantified and histology was performed. SIS+PEDOT group muscles contained less revascularization under the wrap than the SIS group, (p=.05). Histology showed that at 7 days, muscle fibers in the SIS+PEDOT group were not degenerating and regenerating similarly to those muscle fibers in the NONE and SIS groups. Images of SIS and SIS+PEDOT indicate the PEDOT filled the pores of the SIS. This reduced porosity may prohibit revascularization through the SIS+PEDOT. Data indicate that revascularization in the NONE and SIS groups are similar to control muscle while vascularity in the SIS+PEDOT group is impaired by SIS+PEDOT.
doi_str_mv 10.1109/NER.2013.6696220
format conference_proceeding
fullrecord <record><control><sourceid>ieee_CHZPO</sourceid><recordid>TN_cdi_ieee_primary_6696220</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><ieee_id>6696220</ieee_id><sourcerecordid>6696220</sourcerecordid><originalsourceid>FETCH-LOGICAL-i175t-fdbcf34b1209b854dbc87b6de2bccea022e5f08617da5d92d53b61435ea258653</originalsourceid><addsrcrecordid>eNo9kEtLAzEYRaMoWGv3gpssFZya90yWUuoDioroumSSL20knSnJjDj-eisWV_eexbmLi9A5JVNKib55mr9OGaF8qpRWjJEDdEqFKjnVSotDNKJaVAWXUhz9d6FO0CTnD0IIZ0RQXY2Qf2njcMmvRQHdeojQgAvt19CtQ7td7-gKtw12YCHGPpqEszXet9GFZoVD00FK_bbLeJWM78DhTZ9tBJzg02T7K4Rv04W2OUPH3sQMk32O0fvd_G32UCye7x9nt4si0FJ2hXe19VzUlBFdV1LssCpr5YDV1oIhjIH0pFK0dEY6zZzktaKCSzBMVkryMbr42w0AsNymsDFpWO4v4j_jK1sU</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>conference_proceeding</recordtype></control><display><type>conference_proceeding</type><title>Poly(3,4-ethylenedioxythiophene) on decellular scaffolding interrupts grafted muscle revascularization</title><source>IEEE Xplore All Conference Series</source><creator>Baldwin, Joseph F. ; Washabaugh, E. Peter ; Moon, Jana D. ; Shim, Bong-Sup ; Martin, David C. ; Cederna, Paul S. ; Langhals, Nicholas B. ; Urbanchek, Melanie G.</creator><creatorcontrib>Baldwin, Joseph F. ; Washabaugh, E. Peter ; Moon, Jana D. ; Shim, Bong-Sup ; Martin, David C. ; Cederna, Paul S. ; Langhals, Nicholas B. ; Urbanchek, Melanie G.</creatorcontrib><description>Regenerative peripheral nerve interfaces (RPNIs) are implantable devices for amputee communication between peripheral nerves and microprocessor controlled robotic prostheses. Our RPNIs consist of transferred muscles reinnervated by the peripheral nerves remaining in the limb post amputation. RPNI construction includes a light muscle wrap of decellularized small-intestine submucosa (SIS). We studied RPNI revascularization and regeneration when SIS had an electrically conductive coating of poly 3,4 ethylene dioxytheophene (PEDOT). Rats underwent free-muscle transfer (FMT). The peroneal nerve was divided and the proximal end was implanted in the FMT. Experimental groups received either: a) no SIS (NONE), b) SIS (SIS) wrap, or c) wrap of SIS polymerized with PEDOT, (SIS+PEDOT). One week later, rats were perfused with lead oxide, vascular areas were quantified and histology was performed. SIS+PEDOT group muscles contained less revascularization under the wrap than the SIS group, (p=.05). Histology showed that at 7 days, muscle fibers in the SIS+PEDOT group were not degenerating and regenerating similarly to those muscle fibers in the NONE and SIS groups. Images of SIS and SIS+PEDOT indicate the PEDOT filled the pores of the SIS. This reduced porosity may prohibit revascularization through the SIS+PEDOT. Data indicate that revascularization in the NONE and SIS groups are similar to control muscle while vascularity in the SIS+PEDOT group is impaired by SIS+PEDOT.</description><identifier>ISSN: 1948-3546</identifier><identifier>EISSN: 1948-3554</identifier><identifier>EISBN: 1467319694</identifier><identifier>EISBN: 9781467319690</identifier><identifier>DOI: 10.1109/NER.2013.6696220</identifier><language>eng</language><publisher>IEEE</publisher><subject>Educational institutions ; Electrodes ; Muscles ; Optical fiber devices ; Polymers ; Rats ; Surgery</subject><ispartof>2013 6th International IEEE/EMBS Conference on Neural Engineering (NER), 2013, p.1461-1464</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://ieeexplore.ieee.org/document/6696220$$EHTML$$P50$$Gieee$$H</linktohtml><link.rule.ids>309,310,780,784,789,790,2058,27925,54555,54920,54932</link.rule.ids><linktorsrc>$$Uhttps://ieeexplore.ieee.org/document/6696220$$EView_record_in_IEEE$$FView_record_in_$$GIEEE</linktorsrc></links><search><creatorcontrib>Baldwin, Joseph F.</creatorcontrib><creatorcontrib>Washabaugh, E. Peter</creatorcontrib><creatorcontrib>Moon, Jana D.</creatorcontrib><creatorcontrib>Shim, Bong-Sup</creatorcontrib><creatorcontrib>Martin, David C.</creatorcontrib><creatorcontrib>Cederna, Paul S.</creatorcontrib><creatorcontrib>Langhals, Nicholas B.</creatorcontrib><creatorcontrib>Urbanchek, Melanie G.</creatorcontrib><title>Poly(3,4-ethylenedioxythiophene) on decellular scaffolding interrupts grafted muscle revascularization</title><title>2013 6th International IEEE/EMBS Conference on Neural Engineering (NER)</title><addtitle>NER</addtitle><description>Regenerative peripheral nerve interfaces (RPNIs) are implantable devices for amputee communication between peripheral nerves and microprocessor controlled robotic prostheses. Our RPNIs consist of transferred muscles reinnervated by the peripheral nerves remaining in the limb post amputation. RPNI construction includes a light muscle wrap of decellularized small-intestine submucosa (SIS). We studied RPNI revascularization and regeneration when SIS had an electrically conductive coating of poly 3,4 ethylene dioxytheophene (PEDOT). Rats underwent free-muscle transfer (FMT). The peroneal nerve was divided and the proximal end was implanted in the FMT. Experimental groups received either: a) no SIS (NONE), b) SIS (SIS) wrap, or c) wrap of SIS polymerized with PEDOT, (SIS+PEDOT). One week later, rats were perfused with lead oxide, vascular areas were quantified and histology was performed. SIS+PEDOT group muscles contained less revascularization under the wrap than the SIS group, (p=.05). Histology showed that at 7 days, muscle fibers in the SIS+PEDOT group were not degenerating and regenerating similarly to those muscle fibers in the NONE and SIS groups. Images of SIS and SIS+PEDOT indicate the PEDOT filled the pores of the SIS. This reduced porosity may prohibit revascularization through the SIS+PEDOT. Data indicate that revascularization in the NONE and SIS groups are similar to control muscle while vascularity in the SIS+PEDOT group is impaired by SIS+PEDOT.</description><subject>Educational institutions</subject><subject>Electrodes</subject><subject>Muscles</subject><subject>Optical fiber devices</subject><subject>Polymers</subject><subject>Rats</subject><subject>Surgery</subject><issn>1948-3546</issn><issn>1948-3554</issn><isbn>1467319694</isbn><isbn>9781467319690</isbn><fulltext>true</fulltext><rsrctype>conference_proceeding</rsrctype><creationdate>2013</creationdate><recordtype>conference_proceeding</recordtype><sourceid>6IE</sourceid><recordid>eNo9kEtLAzEYRaMoWGv3gpssFZya90yWUuoDioroumSSL20knSnJjDj-eisWV_eexbmLi9A5JVNKib55mr9OGaF8qpRWjJEDdEqFKjnVSotDNKJaVAWXUhz9d6FO0CTnD0IIZ0RQXY2Qf2njcMmvRQHdeojQgAvt19CtQ7td7-gKtw12YCHGPpqEszXet9GFZoVD00FK_bbLeJWM78DhTZ9tBJzg02T7K4Rv04W2OUPH3sQMk32O0fvd_G32UCye7x9nt4si0FJ2hXe19VzUlBFdV1LssCpr5YDV1oIhjIH0pFK0dEY6zZzktaKCSzBMVkryMbr42w0AsNymsDFpWO4v4j_jK1sU</recordid><startdate>201311</startdate><enddate>201311</enddate><creator>Baldwin, Joseph F.</creator><creator>Washabaugh, E. Peter</creator><creator>Moon, Jana D.</creator><creator>Shim, Bong-Sup</creator><creator>Martin, David C.</creator><creator>Cederna, Paul S.</creator><creator>Langhals, Nicholas B.</creator><creator>Urbanchek, Melanie G.</creator><general>IEEE</general><scope>6IE</scope><scope>6IL</scope><scope>CBEJK</scope><scope>RIE</scope><scope>RIL</scope></search><sort><creationdate>201311</creationdate><title>Poly(3,4-ethylenedioxythiophene) on decellular scaffolding interrupts grafted muscle revascularization</title><author>Baldwin, Joseph F. ; Washabaugh, E. Peter ; Moon, Jana D. ; Shim, Bong-Sup ; Martin, David C. ; Cederna, Paul S. ; Langhals, Nicholas B. ; Urbanchek, Melanie G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i175t-fdbcf34b1209b854dbc87b6de2bccea022e5f08617da5d92d53b61435ea258653</frbrgroupid><rsrctype>conference_proceedings</rsrctype><prefilter>conference_proceedings</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Educational institutions</topic><topic>Electrodes</topic><topic>Muscles</topic><topic>Optical fiber devices</topic><topic>Polymers</topic><topic>Rats</topic><topic>Surgery</topic><toplevel>online_resources</toplevel><creatorcontrib>Baldwin, Joseph F.</creatorcontrib><creatorcontrib>Washabaugh, E. Peter</creatorcontrib><creatorcontrib>Moon, Jana D.</creatorcontrib><creatorcontrib>Shim, Bong-Sup</creatorcontrib><creatorcontrib>Martin, David C.</creatorcontrib><creatorcontrib>Cederna, Paul S.</creatorcontrib><creatorcontrib>Langhals, Nicholas B.</creatorcontrib><creatorcontrib>Urbanchek, Melanie G.</creatorcontrib><collection>IEEE Electronic Library (IEL) Conference Proceedings</collection><collection>IEEE Proceedings Order Plan All Online (POP All Online) 1998-present by volume</collection><collection>IEEE Xplore All Conference Proceedings</collection><collection>IEEE Xplore (Online service)</collection><collection>IEEE Proceedings Order Plans (POP All) 1998-Present</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Baldwin, Joseph F.</au><au>Washabaugh, E. Peter</au><au>Moon, Jana D.</au><au>Shim, Bong-Sup</au><au>Martin, David C.</au><au>Cederna, Paul S.</au><au>Langhals, Nicholas B.</au><au>Urbanchek, Melanie G.</au><format>book</format><genre>proceeding</genre><ristype>CONF</ristype><atitle>Poly(3,4-ethylenedioxythiophene) on decellular scaffolding interrupts grafted muscle revascularization</atitle><btitle>2013 6th International IEEE/EMBS Conference on Neural Engineering (NER)</btitle><stitle>NER</stitle><date>2013-11</date><risdate>2013</risdate><spage>1461</spage><epage>1464</epage><pages>1461-1464</pages><issn>1948-3546</issn><eissn>1948-3554</eissn><eisbn>1467319694</eisbn><eisbn>9781467319690</eisbn><abstract>Regenerative peripheral nerve interfaces (RPNIs) are implantable devices for amputee communication between peripheral nerves and microprocessor controlled robotic prostheses. Our RPNIs consist of transferred muscles reinnervated by the peripheral nerves remaining in the limb post amputation. RPNI construction includes a light muscle wrap of decellularized small-intestine submucosa (SIS). We studied RPNI revascularization and regeneration when SIS had an electrically conductive coating of poly 3,4 ethylene dioxytheophene (PEDOT). Rats underwent free-muscle transfer (FMT). The peroneal nerve was divided and the proximal end was implanted in the FMT. Experimental groups received either: a) no SIS (NONE), b) SIS (SIS) wrap, or c) wrap of SIS polymerized with PEDOT, (SIS+PEDOT). One week later, rats were perfused with lead oxide, vascular areas were quantified and histology was performed. SIS+PEDOT group muscles contained less revascularization under the wrap than the SIS group, (p=.05). Histology showed that at 7 days, muscle fibers in the SIS+PEDOT group were not degenerating and regenerating similarly to those muscle fibers in the NONE and SIS groups. Images of SIS and SIS+PEDOT indicate the PEDOT filled the pores of the SIS. This reduced porosity may prohibit revascularization through the SIS+PEDOT. Data indicate that revascularization in the NONE and SIS groups are similar to control muscle while vascularity in the SIS+PEDOT group is impaired by SIS+PEDOT.</abstract><pub>IEEE</pub><doi>10.1109/NER.2013.6696220</doi><tpages>4</tpages></addata></record>
fulltext fulltext_linktorsrc
identifier ISSN: 1948-3546
ispartof 2013 6th International IEEE/EMBS Conference on Neural Engineering (NER), 2013, p.1461-1464
issn 1948-3546
1948-3554
language eng
recordid cdi_ieee_primary_6696220
source IEEE Xplore All Conference Series
subjects Educational institutions
Electrodes
Muscles
Optical fiber devices
Polymers
Rats
Surgery
title Poly(3,4-ethylenedioxythiophene) on decellular scaffolding interrupts grafted muscle revascularization
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T20%3A09%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-ieee_CHZPO&rft_val_fmt=info:ofi/fmt:kev:mtx:book&rft.genre=proceeding&rft.atitle=Poly(3,4-ethylenedioxythiophene)%20on%20decellular%20scaffolding%20interrupts%20grafted%20muscle%20revascularization&rft.btitle=2013%206th%20International%20IEEE/EMBS%20Conference%20on%20Neural%20Engineering%20(NER)&rft.au=Baldwin,%20Joseph%20F.&rft.date=2013-11&rft.spage=1461&rft.epage=1464&rft.pages=1461-1464&rft.issn=1948-3546&rft.eissn=1948-3554&rft_id=info:doi/10.1109/NER.2013.6696220&rft.eisbn=1467319694&rft.eisbn_list=9781467319690&rft_dat=%3Cieee_CHZPO%3E6696220%3C/ieee_CHZPO%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-i175t-fdbcf34b1209b854dbc87b6de2bccea022e5f08617da5d92d53b61435ea258653%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rft_ieee_id=6696220&rfr_iscdi=true