Development of an Immunosensor Based on Layered Double Hydroxides for MMR Cancer Biomarker Detection

• Published in: IEEE transactions on nanobioscience 2015-10, Vol.14 (7), p.688-693
• Main Authors: Hammami, M., Soussou, A., Idoudi, F., Cohen-Bouhacina, T., Bouhaouala-Zahar, B., Baccar, Z. M.
• Format: Magazinearticle
• Language: English
• Subjects: Antibodies; Biomarkers; Biomarkers, Tumor - analysis; Biomarkers, Tumor - immunology; Biotechnology; Cancer; Conductometry - instrumentation; Electrodes; Equipment Design; Equipment Failure Analysis; Feasibility; Feasibility Studies; Humans; Hydroxides; Hydroxides - chemistry; Immune system; Immunoassay - instrumentation; Immunosensor; Immunosensors; layered double hydroxide; Lectins, C-Type - analysis; Lectins, C-Type - immunology; Life Sciences; macrophage mannose receptor; Mannose-Binding Lectins - analysis; Mannose-Binding Lectins - immunology; Nanobioscience; nanomaterials; Neoplasms, Experimental - chemistry; Neoplasms, Experimental - diagnosis; Neoplasms, Experimental - immunology; Receptors, Cell Surface - analysis; Receptors, Cell Surface - immunology; Recombinant; Reproducibility of Results; Sensitivity and Specificity; Spectrum analysis; Surface treatment; Tumors
• ISSN: 1536-1241; 1558-2639
• DOI: 10.1109/TNB.2015.2462025

As a potential biomarker for the investigation of cancer inflammatory profiles, macrophage mannose receptor (MMR, CD206) is herein selected to develop an immunosensor based on layered double hydroxide (LDH). Like an endocyte C-type lectin receptor, MMR plays an important role in immune homeostasis by scavenging unwanted mannose glycoproteins. It attracts a progressive attention thanks to its particularly high expression within the tumor microenvironment. There is a great of interest to develop an immunosensor based on an antibody specific to MMR for detection of stroma versus tumor cells. In this work, we studied the feasibility of high sensitive MMR cancer Screen Printed Electrode (SPE) immunosensor. Working electrode of commercialized SPE was modified by immobilization of specific antibody (anti-MMR) into thin layer of LDH nanomaterials. Structural, morphological, and surface properties of LDHs were studied by X-Ray diffraction, atomic force microscopy and Infrared spectroscopy in ATR. Cyclic Voltammetry technique was used to study interaction between the human recombinant MMR protein (rHu-MMR, NSO derived) and an immobilized antibody into developed immunosensor. High specific response of -11.72 μA/ng·mL -1 (with a correlation coefficient of R 2 =0.994) were obtained in linear range of 0.05 ng/mL to 10.0 ng/mL of specific recombinant antigen. The limit of detection (LOD) was less than 15.0 pg/mL. From these attractive results, the feasibility of an electrochemical immunosensor for cancer was proved. Additional experiments to study stability and reproducibility the immunosensor should be completed in perspective to use these anti-MMR based immunosensors for sensing human MMR in patient biopsies and sera.