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Effects of applied ultrashort pulsed electric fields on solid tumor

Many studies on cancer therapy apply conduct of apoptosis by pulse electric fields have been actively started. In this study, in vivo experiment of applying nanosecond pulse electric fields (nsPEFs) on solid tumors are carried out. To advance this study, we adopt the embryonated chick assay, which c...

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Main Authors:	Nakagawa, Akito, Nagahama, Masataka, Teranishi, Kenji, Uto, Yoshihiro, Shimomura, Naoyuki
Format:	Conference Proceeding
Language:	English
Subjects:	apoptosis Electric fields Electrodes embryonated chick assay Generators nanosecond pulse electric fields Needles Optical pulse generation solid tumor Solids Tumors
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creator	Nakagawa, Akito Nagahama, Masataka Teranishi, Kenji Uto, Yoshihiro Shimomura, Naoyuki
description	Many studies on cancer therapy apply conduct of apoptosis by pulse electric fields have been actively started. In this study, in vivo experiment of applying nanosecond pulse electric fields (nsPEFs) on solid tumors are carried out. To advance this study, we adopt the embryonated chick assay, which can easily prepare a large number of samples for test, compared to typical animal experiments. The tumor cells: EMT6/KU (mouse breast adenocarcinoma cells), were planted with capillary on chorioallantoic membrane in fertilized chicken egg. After four days from this process, nsPEFs of 2ns pulse-width were applied on the solid tumor. The tumors were dissected from eggs after three days from the nsPEFs application and were weighed. The control eggs without nsPEFs application were also processed with the same procedure. Difference of the weights of the pulse-treated and controls tumors was confirmed to be significant by using statistics processing. The weights of the tumors decreased with increase of charging voltage of generator. Tumors treated with nsPEFs exhibited apoptosis by TUNEL staining.
doi_str_mv	10.1109/IPMHVC.2014.7287203
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In this study, in vivo experiment of applying nanosecond pulse electric fields (nsPEFs) on solid tumors are carried out. To advance this study, we adopt the embryonated chick assay, which can easily prepare a large number of samples for test, compared to typical animal experiments. The tumor cells: EMT6/KU (mouse breast adenocarcinoma cells), were planted with capillary on chorioallantoic membrane in fertilized chicken egg. After four days from this process, nsPEFs of 2ns pulse-width were applied on the solid tumor. The tumors were dissected from eggs after three days from the nsPEFs application and were weighed. The control eggs without nsPEFs application were also processed with the same procedure. Difference of the weights of the pulse-treated and controls tumors was confirmed to be significant by using statistics processing. The weights of the tumors decreased with increase of charging voltage of generator. Tumors treated with nsPEFs exhibited apoptosis by TUNEL staining.</abstract><pub>IEEE</pub><doi>10.1109/IPMHVC.2014.7287203</doi><tpages>4</tpages></addata></record>
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subjects	apoptosis Electric fields Electrodes embryonated chick assay Generators nanosecond pulse electric fields Needles Optical pulse generation solid tumor Solids Tumors
title	Effects of applied ultrashort pulsed electric fields on solid tumor
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