Selective Photonic Disinfection of Cell Culture Using a Visible Ultrashort Pulsed Laser

Microbial contamination of cell culture is a major problem encountered both in academic labs and in the biotechnology/pharmaceutical industries. A broad spectrum of microbes, including mycoplasma, bacteria, fungi, and viruses are the causative agents of cell-culture contamination. Unfortunately, the...

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Bibliographic Details
Published in:IEEE journal of selected topics in quantum electronics 2016-05, Vol.22 (3), p.371-378
Main Authors: Tsen, Shaw-Wei D., Kibler, Karen, Jacobs, Bert, Fay, Justin C., Podolnikova, Nataly P., Ugarova, Tatiana P., Achilefu, Samuel, Tsen, Kong-Thon
Format: Article
Language:English
Subjects:
Cell culture
cell cultures
Lasers
Microorganisms
Optical pulse generation
Pathogens
Photonic disinfection
Photonics
Radiation effects
ultrashort pulsed laser
Viruses (medical)
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Summary:Microbial contamination of cell culture is a major problem encountered both in academic labs and in the biotechnology/pharmaceutical industries. A broad spectrum of microbes, including mycoplasma, bacteria, fungi, and viruses are the causative agents of cell-culture contamination. Unfortunately, the existing disinfection techniques lack selectivity and/or lead to the development of drug-resistance, and more importantly there is no universal method to address all the microbes. Here, we report a novel, chemical-free visible ultrashort pulsed laser method for cell-culture disinfection. The ultrashort pulsed laser technology inactivates pathogens with mechanical means, a paradigm shift from the traditional pharmaceutical and chemical approaches. We demonstrate that ultrashort pulsed laser treatment can efficiently inactivate mycoplasma, bacteria, yeast, and viruses with good preservation of mammalian cell viability. Our results indicate that this ultrashort pulsed laser technology has the potential to serve as a universal method for the disinfection of cell culture.
ISSN:1077-260X
1558-4542
DOI:10.1109/JSTQE.2015.2498920