An MCP-based system for beta autoradiography

Describes a pre-commercial prototype autoradiography system which can be used to image a variety of radiolabelled biological samples, ranging from lymphocyte proliferation assays to semi-thin whole body tissue slices. These samples can be imaged with high spatial resolution (/spl sim/70 microns) and...

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Bibliographic Details
Main Authors: Lees, J.E., Pearson, J.F., Fraser, G.W., Hales, J.M., Richards, P.G.
Format: Conference Proceeding
Language:English
Subjects:
Cameras
Detectors
Dynamic range
Electrokinetics
High-resolution imaging
Microchannel
Pixel
Pollution measurement
Prototypes
Spatial resolution
Online Access:Request full text
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Description
Summary:Describes a pre-commercial prototype autoradiography system which can be used to image a variety of radiolabelled biological samples, ranging from lymphocyte proliferation assays to semi-thin whole body tissue slices. These samples can be imaged with high spatial resolution (/spl sim/70 microns) and high sensitivity (e.g. 3H-minimum detectable activity 0.001 Bq in 20 hours) over a wide (5-6 orders of magnitude) dynamic range. Specimens are automatically presented, through a vacuum load-lock, to an imaging microchannel plate (MCP) detector operating in high (/spl sim/10/sup -6/ mbar) vacuum. The authors present images obtained from tritium labelled samples and discuss the importance of the new system for a variety of biological techniques.
ISSN:1082-3654
2577-0829
DOI:10.1109/NSSMIC.1998.773831