Yeast surface displayed porcine epidemic diarrhea (PED) viral epitopes and microfluidic encapsulation in Ca-Alginate beads for oral vaccination

This paper presents the fabrication of the micro fluidic platform for microfluidic encapsulation in Ca-Alginate microspheres toward the whole-cell neutralizing system for PEDV. This system has become a valuable antigen surface exposing tool for a broad spectrum of vaccine development and therapeutic...

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Bibliographic Details
Main Authors: Lee, Ho Won, Kim, Da Som, Lee, Jong Hyun, Kwon, Hyuck Gi, Park, Ga-Young, Ahn, Ji-Young, Jeong, Ok Chan
Format: Conference Proceeding
Language:English
Subjects:
Encapsulation
Fabrication
Fluidic microsystems
Fluorescence
Microfluidics
Microscopy
Oils
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Summary:This paper presents the fabrication of the micro fluidic platform for microfluidic encapsulation in Ca-Alginate microspheres toward the whole-cell neutralizing system for PEDV. This system has become a valuable antigen surface exposing tool for a broad spectrum of vaccine development and therapeutic applications. PED viral epitope regions (ER-1 and ER-2) were successfully expressed on the surface of yeast and their fluorescent activity was observed. Ca-alginate microspheres with encapsulated yeast were successfully produced using the fabricated micro fluidic platform and verified through the microscopic observation of the fluorescent-stained yeast cells in the microsphere.
ISSN:2474-3771
DOI:10.1109/MHS.2017.8305256