Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications

Transport of three different molecules across a porous membrane, with and without a confluent A549 cell monolayer, has been investigated in Transwells and microfluidic devices. The A549 cell line was selected since it has extensively been utilized in toxicology studies due to its potential target fo...

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Bibliographic Details
Main Authors: Frost, Timothy S., Jiang, Linan, Zohar, Yitshak
Format: Conference Proceeding
Language:English
Subjects:
A549 cell line
Biochemistry
convection-diffusion flow
Drugs
Fluorescence
Lung
Media
Microfluidics
pharmacokinetics
protein transport
Stress
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Summary:Transport of three different molecules across a porous membrane, with and without a confluent A549 cell monolayer, has been investigated in Transwells and microfluidic devices. The A549 cell line was selected since it has extensively been utilized in toxicology studies due to its potential target for drug delivery of macro molecules. The measured molecular transport rate was found to decrease with increasing molecular size due to lower diffusivity. The confluent cell monolayer presents a barrier to molecular, significantly reducing the transport rate of larger molecules with little effect on the paracellular transport of smaller molecules. The results indicate that the microfluidic system is a good model for pharmacokinetic applications.
ISSN:2159-6972
DOI:10.1109/NANOMED.2018.8641558