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Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications
Transport of three different molecules across a porous membrane, with and without a confluent A549 cell monolayer, has been investigated in Transwells and microfluidic devices. The A549 cell line was selected since it has extensively been utilized in toxicology studies due to its potential target fo...
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creator | Frost, Timothy S. Jiang, Linan Zohar, Yitshak |
description | Transport of three different molecules across a porous membrane, with and without a confluent A549 cell monolayer, has been investigated in Transwells and microfluidic devices. The A549 cell line was selected since it has extensively been utilized in toxicology studies due to its potential target for drug delivery of macro molecules. The measured molecular transport rate was found to decrease with increasing molecular size due to lower diffusivity. The confluent cell monolayer presents a barrier to molecular, significantly reducing the transport rate of larger molecules with little effect on the paracellular transport of smaller molecules. The results indicate that the microfluidic system is a good model for pharmacokinetic applications. |
doi_str_mv | 10.1109/NANOMED.2018.8641558 |
format | conference_proceeding |
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The results indicate that the microfluidic system is a good model for pharmacokinetic applications.</description><subject>A549 cell line</subject><subject>Biochemistry</subject><subject>convection-diffusion flow</subject><subject>Drugs</subject><subject>Fluorescence</subject><subject>Lung</subject><subject>Media</subject><subject>Microfluidics</subject><subject>pharmacokinetics</subject><subject>protein transport</subject><subject>Stress</subject><issn>2159-6972</issn><isbn>9781538675793</isbn><isbn>153867579X</isbn><fulltext>true</fulltext><rsrctype>conference_proceeding</rsrctype><creationdate>2018</creationdate><recordtype>conference_proceeding</recordtype><sourceid>6IE</sourceid><recordid>eNotkMtOwzAURA0SElXpF8DCP5DgR_xaRqFQpD5YwLq6sW-owU2iJJvy9VRqVzPS6JzFEPLEWc45c8_bcrvbLF9ywbjNrS64UvaGLJyxXEmrjTJO3pKZ4Mpl2hlxTxbj-MMYk9xaYcyMhOoAA_gJh_gX229aqsLRClOi69gihZFCS5d9nA6YIqTLtOnaLsEJh3MLmGjTDfTj7DmC737P2BQ9Lfs-RQ9T7Nrxgdw1kEZcXHNOvl6Xn9UqW-_e3qtynUUh-JQVShah5o1QqgnMQG01kxqYwAaDhAI5rz0zwYfCoxaWQeO0RhmMFdIEJ-fk8eKNiLjvh3iE4bS__iL_AQdiV7E</recordid><startdate>201812</startdate><enddate>201812</enddate><creator>Frost, Timothy S.</creator><creator>Jiang, Linan</creator><creator>Zohar, Yitshak</creator><general>IEEE</general><scope>6IE</scope><scope>6IL</scope><scope>CBEJK</scope><scope>RIE</scope><scope>RIL</scope></search><sort><creationdate>201812</creationdate><title>Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications</title><author>Frost, Timothy S. ; Jiang, Linan ; Zohar, Yitshak</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i221t-4534db1f255fd07ab86036a02efed3a4e11bc07dcd4ce6280af966e3d78237d93</frbrgroupid><rsrctype>conference_proceedings</rsrctype><prefilter>conference_proceedings</prefilter><language>eng</language><creationdate>2018</creationdate><topic>A549 cell line</topic><topic>Biochemistry</topic><topic>convection-diffusion flow</topic><topic>Drugs</topic><topic>Fluorescence</topic><topic>Lung</topic><topic>Media</topic><topic>Microfluidics</topic><topic>pharmacokinetics</topic><topic>protein transport</topic><topic>Stress</topic><toplevel>online_resources</toplevel><creatorcontrib>Frost, Timothy S.</creatorcontrib><creatorcontrib>Jiang, Linan</creatorcontrib><creatorcontrib>Zohar, Yitshak</creatorcontrib><collection>IEEE Electronic Library (IEL) Conference Proceedings</collection><collection>IEEE Proceedings Order Plan All Online (POP All Online) 1998-present by volume</collection><collection>IEEE Xplore All Conference Proceedings</collection><collection>IEEE Electronic Library Online</collection><collection>IEEE Proceedings Order Plans (POP All) 1998-Present</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Frost, Timothy S.</au><au>Jiang, Linan</au><au>Zohar, Yitshak</au><format>book</format><genre>proceeding</genre><ristype>CONF</ristype><atitle>Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications</atitle><btitle>2018 IEEE 12th International Conference on Nano/Molecular Medicine and Engineering (NANOMED)</btitle><stitle>NANOMED</stitle><date>2018-12</date><risdate>2018</risdate><spage>27</spage><epage>30</epage><pages>27-30</pages><eissn>2159-6972</eissn><eisbn>9781538675793</eisbn><eisbn>153867579X</eisbn><abstract>Transport of three different molecules across a porous membrane, with and without a confluent A549 cell monolayer, has been investigated in Transwells and microfluidic devices. The A549 cell line was selected since it has extensively been utilized in toxicology studies due to its potential target for drug delivery of macro molecules. The measured molecular transport rate was found to decrease with increasing molecular size due to lower diffusivity. The confluent cell monolayer presents a barrier to molecular, significantly reducing the transport rate of larger molecules with little effect on the paracellular transport of smaller molecules. The results indicate that the microfluidic system is a good model for pharmacokinetic applications.</abstract><pub>IEEE</pub><doi>10.1109/NANOMED.2018.8641558</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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identifier | EISSN: 2159-6972 |
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source | IEEE Xplore All Conference Series |
subjects | A549 cell line Biochemistry convection-diffusion flow Drugs Fluorescence Lung Media Microfluidics pharmacokinetics protein transport Stress |
title | Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications |
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