A precision technology for controlling protein adsorption and cell adhesion in bioMEMS

A surface coating technique is investigated to enhance device biocompatibility by eliminating bio-fouling, the strong but non-specific affinity of proteins and cells to attach to surfaces. This coating is a conformal, thin poly(ethylene glycol)-like film deposited in a glow discharge of tetraglyme....

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Bibliographic Details
Main Authors: Pan, Y.V., Hanein, Y., Leach-Scampavia, D., Bohringer, K.F., Ratner, B.D., Denton, D.D.
Format: Conference Proceeding
Language:English
Subjects:
Adhesives
Biosensors
Chemical and biological sensors
Coatings
Immune system
Plasmas
Polymers
Proteins
Surface discharges
Surface resistance
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Summary:A surface coating technique is investigated to enhance device biocompatibility by eliminating bio-fouling, the strong but non-specific affinity of proteins and cells to attach to surfaces. This coating is a conformal, thin poly(ethylene glycol)-like film deposited in a glow discharge of tetraglyme. Substrates with different chemistries are successfully modified, and exhibit ultralow protein adsorption and cell attachment with the coating. This "stealth" or "non-fouling" coating can also be faithfully patterned using standard photolithography processes. The interaction of proteins and cells with patterned surfaces is limited only to the protein-adhesive domains, thus creating heterogeneous patterns of proteins and cell cultures on the surface. The potential benefits of our technique to applications such as cell-based assays and micro-electrodes are discussed.
ISSN:1084-6999
DOI:10.1109/MEMSYS.2001.906571