Nanomaterial-Enhanced Fiber Optofluidic Laser Biosensor for Sensitive Enzyme Detection

A novel fiber optofluidic laser biosensor based on nanoparticle-functionalized thin-walled hollow optical fiber is developed for sensitive detection of enzyme horseradish peroxidase (HRP). High sensitivity is achieved thanks to the high surface-to-volume ratio of the nanomaterials. Two kinds of attr...

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Bibliographic Details
Published in:Journal of lightwave technology 2020-09, Vol.38 (18), p.5205-5211
Main Authors: Mao, Jiangui, Yang, Xi, Liu, Yiling, Wang, Yanqiong, Peng, Gang-Ding, Rao, Yun-Jiang, Gong, Yuan
Format: Article
Language:English
Subjects:
Biochemistry
Biomolecules
Biosensor
Biosensors
Biotin
Conjugation
Crosslinking
Enzymes
Fiber lasers
Gold
Lasers
Linearity
nanomaterial
Nanomaterials
Nanoparticles
Nanorods
optical fiber sensor
Optical fiber sensors
Optical fibers
Optical surface waves
optofluidic laser
Peroxidase
Sensitivity enhancement
Silicon dioxide
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Summary:A novel fiber optofluidic laser biosensor based on nanoparticle-functionalized thin-walled hollow optical fiber is developed for sensitive detection of enzyme horseradish peroxidase (HRP). High sensitivity is achieved thanks to the high surface-to-volume ratio of the nanomaterials. Two kinds of attractive nanomaterials, i.e., Au nanorods and SiO 2 nanoparticles, are selected as demonstrative models to be coated onto the inner surface of thin-walled hollow fiber through electrostatic adsorption and biochemical cross-linking, respectively, while the streptavidin-biotin conjugation mechanism acts as a bridge for the specific recognition of enzyme. The nanoparticle-functionalized fiber optofluidic lasers have lower lasing threshold, compared to the control group without nanomaterial. We experimentally demonstrate that the nanomaterials enhanced fiber optofluidic laser biosensors can detect HRP in the range of 75-15 000 pM with a good linearity in semi-logarithmic scale, and with a sensitivity enhancement of 57.4% by using the SiO 2 nanoparticles. We believe that this nanoparticle-enhanced fiber optofluidic laser biosensor could provide an efficient strategy for biomolecule detection such as enzyme-linked immunosorbent assay (ELISA).
ISSN:0733-8724
1558-2213
DOI:10.1109/JLT.2020.2997993