Laser-induced fluorescent diagnostics and photodynamic therapy of cervical neoplasms

• Main Authors: Alekseeva, P.M., Efendiev, K.T., Shiryaev, A.A., Amirkhanova, L.M., Linkov, K.G., Loschenov, V.B.
• Format: Conference Proceeding
• Language: English
• Subjects: cervical neoplasms; fluorescent diagnostics; photobleaching; photodynamic therapy
• ISSN: 2642-5580
• DOI: 10.1109/ICLO48556.2020.9285518

The paper presents the results of precise fluorescent diagnostics of cervical neoplasms at photodynamic therapy using a photosensitizer, chlorin e6 (Photolon). The precise fluorescent diagnostics supposes the combined use of spectral and video fluorescent diagnostics of pathological and normal tissues. A fiber spectrometer with excitation of the photosensitizer fluorescence by a He-Ne laser (λ = 632.8 nm) and a semiconductor laser (λ = 660 nm) was used for spectral fluorescent diagnostics. A two-channel fluorescent video system was used for video fluorescent diagnostics. The results of spectral diagnostics showed that the chlorin e6 accumulation level in pathological tissues is 4- 8 times higher than in normal tissues. The fluorescent images of cervical neoplasms at the applied dose of the photosensitizer had high contrast compared to normal tissues. The results of spectral fluorescent diagnostics well correlate with the results of video fluorescent diagnostics. Photodynamic therapy was performed using the semiconductor laser (λ = 660 nm, E = 100-200 J/cm 2 ) 3 hours after a drip intravenous administration of the photosensitizer and was controlled via photobleaching.