Dynamics of PpIX accumulation in A549, HeLa and 3T3 cell lines

The synthesis and accumulation of protoporphyrin IX (PpIX), induced by 5-aminolevulinic acid (5-ALA) in HeLa, A549 and 3T3 cells was studied using confocal fluorescence microscopy. The accumulation dynamics of PpIX in cells was determined from the kinetics of its fluorescence intensity. The PpIX syn...

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Bibliographic Details
Main Authors: Gorbenko, D.A., Belashov, A.V., Belyaeva, T.N., Kornilova, E.S., Semenova, I.V., Vasyutinskii, O.S.
Format: Conference Proceeding
Language:English
Subjects:
5-aminolevulinic acid
confocal fluorescence microscopy
photodynamic therapy
protoporphyrin IX
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Summary:The synthesis and accumulation of protoporphyrin IX (PpIX), induced by 5-aminolevulinic acid (5-ALA) in HeLa, A549 and 3T3 cells was studied using confocal fluorescence microscopy. The accumulation dynamics of PpIX in cells was determined from the kinetics of its fluorescence intensity. The PpIX synthesis rate was analyzed as function of 5-ALA concentration in the culture medium and incubation time.
ISSN:2642-5580
DOI:10.1109/ICLO48556.2020.9285888