Further studies on selection for viable cells in culture using cell sorter technology

The authors have developed a two-channel method, using forward and lateral light scatter, for selecting live and nonviable cultured lymphoblastoid cells without exposing the cells to any dye. These parameters are related to cell size and cell granularity, both of which are quickly altered in cells w...

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Bibliographic Details
Main Authors: Tanz, W.S., Tsongalis, G.J., Lamber, W.C.
Format: Conference Proceeding
Language:English
Subjects:
Batteries
Cells (biology)
Cloning
DNA
Genetics
Humans
In vitro
Light scattering
Pathology
Testing
Online Access:Request full text
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Summary:The authors have developed a two-channel method, using forward and lateral light scatter, for selecting live and nonviable cultured lymphoblastoid cells without exposing the cells to any dye. These parameters are related to cell size and cell granularity, both of which are quickly altered in cells which become nonviable as determined by dye exclusion (0.1% trypan blue). Using the nontoxic vital dye, fluorescein diacetate, the authors have also developed a system which only requires a single channel to unequivocally separate live and nonviable lymphoblastoid cells in culture.< >
DOI:10.1109/IEMBS.1988.94529