A Microfluidic Chip for Growth and Characterization of Adult Rat Hippocampal Progenitor Cell Neurospheroids

Adult hippocampal neural stem/progenitor cells (AHPCs), which are self-renewing multipotent progenitors that can differentiate into neurons, astrocytes, and oligodendrocytes, are suitable as a central nervous system (CNS) molecular model, and the formation of 3D AHPC neurospheroids are potentially s...

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Bibliographic Details
Published in:Journal of microelectromechanical systems 2022-02, Vol.31 (1), p.37-44
Main Authors: Yang, Renyuan, Fonder, Catherine, Sylvester, Talia, Peng, Stefan, Jiles, David, Sakaguchi, Donald S., Que, Long
Format: Article
Language:English
Subjects:
Adult rat hippocampal progenitor cells (AHPCs)
Brain
Brain modeling
Cells (biology)
Central nervous system
Chambers
differentiation
Diseases
Fluorescence
In vitro
microfluidic chip
Microfluidics
neurospheroids
proliferation
Resists
Shape
Solid modeling
Three-dimensional displays
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Summary:Adult hippocampal neural stem/progenitor cells (AHPCs), which are self-renewing multipotent progenitors that can differentiate into neurons, astrocytes, and oligodendrocytes, are suitable as a central nervous system (CNS) molecular model, and the formation of 3D AHPC neurospheroids are potentially suitable as an in vitro brain model. In this paper we report a new microfluidic chip to culture AHPC neurospheroids (NSs-AHPC) inside culture-chambers on chip. After cell fixation and immunostaining were conducted, the fluorescence images of NSs-AHPC were analyzed. It has been found that the AHPCs comprising neurospheroids remained highly viable. Cell proliferation and neuronal differentiation have also been observed, indicating the feasibility of NSs-AHPC as the in vitro brain model on chip. Given its simple-to-use, low-cost, and orderly arranged culture-chambers, this type of chip is particularly suitable for culturing and analyzing multiple in vitro brain models in an efficient manner. [2021-0199]
ISSN:1057-7157
1941-0158
DOI:10.1109/JMEMS.2021.3134632