Microfluidic Platform to Study Electric Field Based Root Targeting by Pathogenic Zoospores

This paper reports the fabrication and application of a microfluidic Lab-on-a-Chip platform to study the electrotactic movements of pathogenic microorganisms. The movement of the pathogens in response to electric fields are one way in which they are thought to locate their hosts. Design and fabricat...

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Bibliographic Details
Main Authors: Sarkar, Debolina, Sun, Yiling, Tayagui, Ayelen, Adams, Ryan, Garrill, Ashley, Nock, Volker
Format: Conference Proceeding
Language:English
Subjects:
Conferences
Electric Fields
Electric potential
Electrodes
Electrotaxis
Fabrication
Lab-on-a-Chip
Microorganisms
Pathogens
Phytophthora nicotianae
Voltage
Zoospores
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Summary:This paper reports the fabrication and application of a microfluidic Lab-on-a-Chip platform to study the electrotactic movements of pathogenic microorganisms. The movement of the pathogens in response to electric fields are one way in which they are thought to locate their hosts. Design and fabrication of the platform, and associated micro-electronics are described. The platform contains arrays of micro-electrodes that generate an electric field of defined strength in a micro-chamber into which feed inlet and outlet channels for entry and exit of media and microorganisms. To demonstrate applicability of the platform, motile zoospores of the pathogenic oomycete Phytophthora nicotianae were seeded in the inlet and a voltage was applied to investigate the electrotactic responses of the zoospores. This platform offers a unique opportunity to study electrotactic movements that may be responsible for the ability of the pathogens to locate and invade host tissue.
ISSN:2160-1968
DOI:10.1109/MEMS51670.2022.9699559