Evaluation of therapeutic potential of nanosilver particles synthesised using aloin in experimental murine mastitis model

• Published in: IET nanobiotechnology 2013-09, Vol.7 (3), p.78-82
• Main Authors: Chaitanya Kumar, Thota Venkata, Muralidhar, Yegireddy, Prasad, Pagadala Eswara, Prasad, Tollamadugu Naga Venkata Krishna Vara, Alpha Raj, Mekapogu
• Format: Article
• Language: English
• Subjects: aloin mediated nanosilver particle synthesis; Animals; antibiotic cefepime; Antioxidants - metabolism; biochemistry; blunt Hamilton needle; Body Weight; broth culture; catalase activity; cellular biophysics; Disease Models, Animal; diseases; Drug Design; drugs; Emodin - analogs & derivatives; Emodin - therapeutic use; Female; histopathology; in vitro spleenocyte viability; intramammary routes; intraperitoneal routes; ketamine anaesthesia; mammary gland bacterial load; Mastitis - drug therapy; Mastitis - microbiology; Metal Nanoparticles - therapeutic use; Mice; microorganisms; molecular biophysics; murine mastitis model; nanobiotechnology; nanofabrication; nanomedicine; nanoparticles; organic compounds; patient treatment; Phylogeny; polymorpho nuclear cell infiltration; post inoculation; proteins; reduced glutathione content; reduction (chemical); S. aureus; serum C reactive protein; Silver - therapeutic use; silver nitrate reduction; Special Issue: Recent Advances in Biosynthesis of Nanoparticles and their Applications; Staphylococcal Infections - drug therapy; Staphylococcus aureus; superoxide dismutase activity; therapeutic potential evaluation; thiobarbituric acid reactive substance content; time 18 h; time 24 h; time 6 h
• ISSN: 1751-8741; 1751-875X; 1751-875X
• DOI: 10.1049/iet-nbt.2012.0045

Nanobiotechnology is an emerging biological branch of nanotechnology. Application of nanoparticles with specific size and shape in biology has already shown unforeseen and interesting results. A study was conducted to evaluate the therapeutic potential of phytogenically derived aloin mediated nanosilver particles (AAgNPs), prepared by reduction of silver nitrate with aloin, in Staphylococcus aureus induced murine mastitis. A total of 40 female mice were divided into five groups of eight animals each. Group I served as lactating control, groups II-V were inoculated with 20 μl of 24 h broth culture of S. aureus containing 4.0 × 105 cfu/quarter under ketamine anaesthesia. After 6 h post inoculation, groups III and IV received 20 μl of aloin nanosilver (AAgNPs) through intramammary and intraperitoneal routes, respectively. Group V received antibiotic cefepime at 1 mg/kg body weight through the intra-peritoneal route. After 18 h post-treatment, serum C reactive protein, weights of mammary glands, mammary gland bacterial load, thiobarbituric acid reactive substances content, reduced glutathione content, superoxide dismutase activity and catalase activity and histopathology were determined. The compound showed a minimum inhibitory concentration of 21.8 ng/ml against S. aureus. Significant reduction (98%) in poly-morpho nuclear cell infiltration was observed with AAgNPs than antibiotic (50%).