Development and Validation of Stability-Indicating RP-HPLC Method for Determination of Metoprolol Succinate and Olmesartan Medoxomil in Bulk and in Formulation

An accurate, precise, robust and economic stability-indicating RP HPLC method for determination of Metoprolol succinate and Olmesartan medoxomil in bulk and in formulation has been developed and validated. Separation was carried out on HiQSil C18 column (250 x 4.6 mm, 5 μm) at flow rate of 1 ml/min...

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Bibliographic Details
Published in:Research journal of pharmacy and technology 2014-12, Vol.7 (12), p.1368-1373
Main Authors: Singh, Anamika, Dwivedi, Jaya, Gandhi, Santosh V
Format: Article
Language:English
Subjects:
Metoprolol succinate
Olmesartan medoxomil
RP HPLC
Stability
Online Access:Get full text
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Summary:An accurate, precise, robust and economic stability-indicating RP HPLC method for determination of Metoprolol succinate and Olmesartan medoxomil in bulk and in formulation has been developed and validated. Separation was carried out on HiQSil C18 column (250 x 4.6 mm, 5 μm) at flow rate of 1 ml/min using Methanol: 10 mM ophosphoric acid buffer (50: 50 v/v) and detection at 216 nm. On a stabilized chromatographic condition Metoprolol succinate and Olmesartan medoxomil gave acceptable peak at RT 3.66 ± 0.04 min and 18.14 ± 0.05 min, respectively. The calibration curve for Metoprolol succinate and Olmesartan medoxomil was found to be linear in the range 1–25 μg/ml and 1–10 μg/ml, respectively. The method was validated in accordance with international conference on harmonization (ICH) guidelines and prescribed stress conditions. The drugs were subjected to stress condition of hydrolysis (acid, base, neutral), oxidation, photolysis and thermal degradation. The proposed method can be applicable for the routine determination of Metoprolol succinate and Olmesartan medoxomil in bulk and formulation.
ISSN:0974-3618
0974-360X