Vacuolating Toxin Gene Polymorphism among Helicobacter pylori Clinical Isolates and Its Association with m1, m2, or Chimeric vacA Middle Types

Background: Helicobacter pylori vacuolating cytotoxin encoded by vacA plays an essential role in H. pylori-related pathogenesis. Specific vacA alleles are believed to be associated with increased virulence. Association among vacA polymorphism, vacA middle genotypes, and various H. pylori-related dis...

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Published in:Scandinavian journal of gastroenterology 1998, Vol.33 (11), p.1152-1157
Main Authors: YANG, J. C, KUO, C. H, WANG, H. J, WANG, T. C, CHANG, C. S, WANG, W. C
Format: Article
Language:English
Subjects:
Bacterial diseases
Bacterial diseases of the digestive system and abdomen
Bacterial Proteins - genetics
Biological and medical sciences
Cytotoxins - genetics
Female
Gastrointestinal Diseases - microbiology
Genetic Variation
Genotype
Helicobacter Infections - microbiology
Helicobacter pylori - genetics
Helicobacter pylori - isolation & purification
Human bacterial diseases
Humans
Infectious diseases
Male
Medical sciences
Middle Aged
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
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Summary:Background: Helicobacter pylori vacuolating cytotoxin encoded by vacA plays an essential role in H. pylori-related pathogenesis. Specific vacA alleles are believed to be associated with increased virulence. Association among vacA polymorphism, vacA middle genotypes, and various H. pylori-related diseases was thus investigated. Methods: Eighty-nine isolates from patients with various gastrointestinal diseases were examined for restriction fragment length polymorphism (RFLP) of the 2.0-kb polymerase chain reaction-amplified vacA middle region. Further genetic heterogeneity was assessed with ureA-ureB RFLP. Results: Twenty-eight distinct vacA RFLPs were seen among 89 isolates. Each pattern was associated with one specific vacA middle genotype. The association of specific RFLPs with certain clinical manifestations was noted among six common groups. Further RFLP analysis of the 2.4-kb ureA-ureB segment from isolates in four popular vacA RFLPs showed high genetic variation. Conclusions: The vacA genetic polymorphism may be associated with different gastrointestinal diseases.
ISSN:0036-5521
1502-7708
DOI:10.1080/00365529850172494