13C Mixed-Triglyceride Breath Test: Isotope Selective Non-Dispersive Infrared Spectrometry in Comparison with Isotope Ratio Mass Spectrometry in Volunteers and Patients with Chronic Pancreatitis

Background: The 13C mixed-triglyceride breath test (MTB) has been proposed for the non-invasive assessment of duodenal pancreatic lipase activity. Until now, stable isotope analysis of CO2 of the MTB has been carried out with isotope ratio mass spectrometry (IRMS). The aim of the present study was t...

Full description

Saved in:
Bibliographic Details
Published in:Scandinavian journal of gastroenterology 1999-11, Vol.34 (11), p.1153-1156
Main Author: Boedeker, C.
Format: Article
Language:English
Subjects:
Adult
Aged
Biological and medical sciences
Breath Tests - methods
Carbon Dioxide - analysis
Carbon Isotopes
Chronic Disease
Digestion. Liver. Biliary tract. Spleen. Pancreas
Female
Humans
Investigative techniques, diagnostic techniques (general aspects)
Linear Models
Lipase - metabolism
Male
Medical sciences
Middle Aged
Pancreas - enzymology
Pancreatitis - diagnosis
Pancreatitis - enzymology
Radionuclide investigations
Spectrophotometry, Infrared - methods
Triglycerides - pharmacokinetics
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background: The 13C mixed-triglyceride breath test (MTB) has been proposed for the non-invasive assessment of duodenal pancreatic lipase activity. Until now, stable isotope analysis of CO2 of the MTB has been carried out with isotope ratio mass spectrometry (IRMS). The aim of the present study was to compare MTB results by using the new non-dispersive infrared spectrometry (NDIRS) and the IRMS. Methods: Ten healthy volunteers and 10 patients with chronic pancreatitis and exocrine insufficiency were studied. After an overnight fast each subject received a test meal containing 250 mg 1,3 distearyl, 2[13C] octanoyl glycerol. Breath samples were taken at base line and at 30-min intervals over a period of 6 h postprandially. The 13C/12C ratio was determined in each breath sample by NDIRS and CF-IRMS as delta values. Results were expressed as delta over base line (DOB (?)) and as cumulative percentage dose of 13
ISSN:0036-5521
1502-7708
DOI:10.1080/003655299750024977