An inhibitory monoclonal antibody to human cytochrome P450 that specifically binds and inhibits P4502C9II, an allelic variant of P4502C9 having a single amino acid change Arg144 Cys

• Published in: Xenobiotica 2000-06, Vol.30 (6), p.619-625
• Main Authors: Krausz, K. W., Goldfarb, I., Yang, T. J., Gonzalez, F. J., Gelboin, H. V.
• Format: Article
• Language: English
• Subjects: Alleles; Amino Acid Substitution - genetics; Analytical, structural and metabolic biochemistry; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - pharmacology; Antibody Specificity; Arginine - genetics; Arginine - metabolism; Aryl Hydrocarbon Hydroxylases; Biological and medical sciences; Cysteine - genetics; Cysteine - metabolism; Cytochrome P-450 Enzyme Inhibitors; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - immunology; Cytochrome P-450 Enzyme System - metabolism; cytochrome P450; Diclofenac - metabolism; Enzyme-Linked Immunosorbent Assay; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Genetic Variation - genetics; Humans; Hybridomas; Isoenzymes - antagonists & inhibitors; Isoenzymes - genetics; Isoenzymes - immunology; Isoenzymes - metabolism; Oxidoreductases; Phenanthrenes - metabolism; Polymorphism, Genetic - genetics; Polymorphism, Genetic - immunology; Protein Binding - genetics; Protein Binding - immunology; Recombinant Proteins - antagonists & inhibitors; Recombinant Proteins - immunology; Recombinant Proteins - metabolism; Steroid 16-alpha-Hydroxylase; Steroid Hydroxylases - antagonists & inhibitors; Steroid Hydroxylases - genetics; Steroid Hydroxylases - immunology; Steroid Hydroxylases - metabolism; Tolbutamide - metabolism
• ISSN: 0049-8254; 1366-5928
• DOI: 10.1080/004982500406444

A monoclonal antibody (MAb 292-2-3) has been isolated that binds specifically to a single allele of three expressed human cytochrome P4502C9 alleles. The MAb binds to 2C9