Increased Inflammatory Factors Activity in Model Rats with Experimental Autoimmune Prostatitis

Male rats were immunized with prostate tissue homogenate supernate (PTHS) of male rats with complete Freund's adjuvant (CFA) intra dermal in the multiple points and simultaneously immunized with 0.5 ml Pertussis-Diphtheria-Tetanus (PDT) vaccine intra peritonea on 0 and 30th day. At the 45th day...

Full description

Saved in:
Bibliographic Details
Published in:Archives of andrology 2007-01, Vol.53 (2), p.49-52
Main Authors: Zhou, Xiao-hui, Li, Lian-da, Wu, Li-mao, Han, Lei, Liu, Zhong-de, Yang, Ji-xiang, Lv, Yan-wei, You, Chun-lai, Zhou, Zhi-heng
Format: Article
Language:English
Subjects:
Animals
Autoimmune Diseases - pathology
Autoimmune Diseases - physiopathology
chronic abacterial prostatitis
Disease Models, Animal
immune
In Situ Hybridization
Inflammation - physiopathology
iNOS
Male
Nitric Oxide Synthase Type II - genetics
Prostatitis - immunology
Prostatitis - pathology
Prostatitis - physiopathology
Rats
Rats, Wistar
TNF-α
Tumor Necrosis Factor-alpha - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Male rats were immunized with prostate tissue homogenate supernate (PTHS) of male rats with complete Freund's adjuvant (CFA) intra dermal in the multiple points and simultaneously immunized with 0.5 ml Pertussis-Diphtheria-Tetanus (PDT) vaccine intra peritonea on 0 and 30th day. At the 45th day after first immunization, animals were sacrificed and a series of examinations such as HE stain, assay of TNF-α by ELISA and assay of inducible nitric oxide synthase (iNOS) mRNA by in-situ hybridization (ISH) were taken. We observed that there was a remarkable up-regulation of TNF-α expression in the high dosage model group. The results of macropathology, histopathology and iNOS ISH also revealed the same tendency. This experimental procedure is effective to induce chronic abacterial prostatitis (CAP).
ISSN:0148-5016
1521-0375
DOI:10.1080/01485010600908397