Gene Transfer to Trabecular Meshwork Endothelium via Direct Injection into the Schlemm Canal and In Vivo Toxicity Study

Purpose: Our aim was to investigate the efficiency of adenoviral gene transfer via direct injection into the Schlemm canal ex vivo in human donor eyes and to examine the effect of human MMP-3 transgene expression in a rat model in vivo. Methods: A viscocanalostomy-like operation was performed and ad...

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Bibliographic Details
Published in:Current eye research 2005-12, Vol.30 (12), p.1051-1059
Main Authors: Hudde, Tobias, Apitz, Janine, Bordes-Alonso, Rafael, Heise, Kerstin, Johnson, Kristian T. M., Steuhl, Klaus-Peter, Geerling, Gerd, Pützer, Brigitte M.
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
Animals
Anterior Chamber - enzymology
Anterior Chamber - virology
Blotting, Western
Cell Line
Endothelium - enzymology
Endothelium - pathology
Endothelium, Corneal - enzymology
eye
Gene Expression Regulation, Enzymologic - physiology
gene transfer
Gene Transfer Techniques
Genes, Reporter
Genetic Vectors
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Humans
Injections
Limbus Corneae - enzymology
Limbus Corneae - virology
Male
matrix metalloprotease 3
Matrix Metalloproteinase 3 - genetics
Matrix Metalloproteinase 3 - metabolism
Rats
Rats, Sprague-Dawley
trabecular meshwork
Trabecular Meshwork - enzymology
Trabecular Meshwork - pathology
Transgenes
viscocanalostomy
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Summary:Purpose: Our aim was to investigate the efficiency of adenoviral gene transfer via direct injection into the Schlemm canal ex vivo in human donor eyes and to examine the effect of human MMP-3 transgene expression in a rat model in vivo. Methods: A viscocanalostomy-like operation was performed and adenoviral vector encoding for MMP-3 and green fluorescent protein was injected into human Schlemm canal or rat anterior chamber. Results: Transgene expression was high in trabecular meshwork endothelium in human donor eyes. In vivo, adenovirus caused dose-dependent inflammation. Conclusions: Direct injection of adenoviral vectors into the Schlemm canal has potential in glaucoma treatment.
ISSN:0271-3683
1460-2202
DOI:10.1080/02713680500323350