Purification of an antimicrobial peptide from rabbit aqueous humour

Purpose. To identify an antimicrobial factor previously demonstrated in rabbit aqueous humour. Methods. Rabbit aqueous humour was fractionated by a multistage process involving anion-exchange and size-exclusion liquid chromatography. The antimicrobial effect of aqueous humour fractions upon Staphylo...

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Bibliographic Details
Published in:Current eye research 1998-08, Vol.17 (8), p.783-787
Main Authors: Diamond, Jeremy P., Moule, Kelly, Leeming, John P., Tavare, Jeremy, Easty, David L.
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - isolation & purification
Anti-Bacterial Agents - pharmacology
antimicrobial peptide
Aqueous Humor - chemistry
aqueous humour
Chromatography, Gel
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Colony Count, Microbial
Electrophoresis, Polyacrylamide Gel
Molecular Weight
peptide purification
Peptides
rabbit
Rabbits
Staphylococcus aureus
Staphylococcus aureus - drug effects
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Summary:Purpose. To identify an antimicrobial factor previously demonstrated in rabbit aqueous humour. Methods. Rabbit aqueous humour was fractionated by a multistage process involving anion-exchange and size-exclusion liquid chromatography. The antimicrobial effect of aqueous humour fractions upon Staphylococcus aureus were evaluated in an in vitro model. The components of aqueous humour fractions mediating an antimicrobial effect were investigated by SDS-PAGE. Results. A single peptide of molecular weight approximately 8 kDa was identified which mediated an antimicrobial effect upon Staphylococcus aureus. Attempts to identify the peptide have been unsuccessful. Conclusions. Rabbit aqueous humour contains an unidentified peptide that mediates an antimicrobial effect upon Staphylococcus aureus. If such a peptide is present in human aqueous humour it may contribute to the apparent resistance to bacterial infection manifest in the anterior chamber. Curr. Eye Res. 17: 783-787, 1998.
ISSN:0271-3683
1460-2202
DOI:10.1080/02713689808951258