HB-GAM/Pleiotrophin and Midkine are Differently Expressed and Distributed During Retinoic Acid-induced Neural Differentiation of P19 Cells

HB-GAM/Pleiotrophin and Midkine (MK) are developmentally-regulated proteins with putative functions during cell growth and differentiation. Using the P19 cell which is a model to study the events associated with early development, we examined the expression and cellular localization of HB-GAM and MK...

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Bibliographic Details
Published in:Growth factors (Chur, Switzerland) Switzerland), 2003-09, Vol.21 (3-4), p.139-149
Main Authors: Brunet-De Carvalho, Nicole, Raulais, Daniel, Rauvala, Heikki, Souttou, Boussad, Vigny, Marc
Format: Article
Language:English
Subjects:
Animals
Blotting, Northern
Carcinoma, Embryonal - metabolism
Carrier Proteins - biosynthesis
Cell Differentiation
Cell Line
Cell Line, Tumor
Choline O-Acetyltransferase - metabolism
Cytokines - biosynthesis
DNA - chemistry
DNA - metabolism
DNA, Complementary - metabolism
Embryonal carcinoma cells
Extracellular matrix
Extracellular Matrix - metabolism
HB-GAM
Humans
Immunohistochemistry
Keratolytic Agents - pharmacology
Membrane Glycoproteins - biosynthesis
Mice
Midkine
Neuronal differentiation
Neurons - cytology
Neurons - metabolism
Proteoglycans - biosynthesis
Recombinant Proteins - chemistry
RNA, Messenger - metabolism
Syndecan-3
Time Factors
Tretinoin - metabolism
Tretinoin - pharmacology
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Summary:HB-GAM/Pleiotrophin and Midkine (MK) are developmentally-regulated proteins with putative functions during cell growth and differentiation. Using the P19 cell which is a model to study the events associated with early development, we examined the expression and cellular localization of HB-GAM and MK during neural differentiation of P19 cells induced by retinoic acid (RA). The temporal expressions of HB-GAM and MK transcripts and both the levels and cellular localizations of the corresponding proteins appeared dramatically different. MK mRNA, already expressed in untreated P19 cells, was transiently increased by exposure to RA and then largely down regulated. More interestingly, HB-GAM which was not detected in untreated P19 cells was strongly expressed after 2 days of RA treatment and this expression persists throughout the duration of the culture suggesting that it could be involved in different aspects of this differentiation process.
ISSN:0897-7194
1029-2292
DOI:10.1080/08977190310001621014