Effect of signal transduction inhibition in adapted lymphocytes: micronuclei frequency and DNA repair

Irradiation of human lymphocytes (1 cGy X rays, 37 C) or their treatment with 10 mu m hydrogen peroxide (30 min at 37 C) evoked a ca 30% decrease in the frequency of micronuclei upon subsequent X-irradiation (1.5 Gy). The response was reflected in a lower micronuclei frequency, but no change in DNA...

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Bibliographic Details
Published in:International journal of radiation biology 1997-03, Vol.71 (3), p.245-252
Main Author: WOJEWODZKA, M. KRUSZEWSKI and I. SZUMIEL, M.
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Adult
Biological and medical sciences
Calcium - antagonists & inhibitors
Calcium Channel Blockers - pharmacology
DNA Repair - drug effects
Enzyme Inhibitors - pharmacology
Fundamental and applied biological sciences. Psychology
Gallic Acid - analogs & derivatives
Gallic Acid - pharmacology
Humans
Male
Micronucleus Tests
Molecular and cellular biology
Molecular genetics
Mutagenesis. Repair
Protein Kinase Inhibitors
Signal Transduction - drug effects
Space life sciences
Staurosporine - pharmacology
X-Rays
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Summary:Irradiation of human lymphocytes (1 cGy X rays, 37 C) or their treatment with 10 mu m hydrogen peroxide (30 min at 37 C) evoked a ca 30% decrease in the frequency of micronuclei upon subsequent X-irradiation (1.5 Gy). The response was reflected in a lower micronuclei frequency, but no change in DNA repair rate was observed as measured by the comet assay, directly after the challenge dose. Treatment of lymphocytes with staurosporine, an inhibitor of protein kinases, or with TMB-8, a calcium antagonist, carried out in parallel with the adaptive dose prevented the development of the adaptive response measured as micronuclei frequency. In lymphocytes that were staurosporine- or TMB-8-treated and irradiated under adaptive conditions showed that the rate of DNA repair was not changed. We conclude that treatment with agents that interfere with the transduction of the signal triggered by the low dose prevents the development of the adaptive response induced by X rays or hydrogen peroxide. Lower chromosome damage revealed by the cytokinesis block-micronuclei test in the adapted lymphocytes is unrelated to DNA repair rate as measured by comet assay.
ISSN:0955-3002
1362-3095
DOI:10.1080/095530097144111