Cloning and Characterization of a New Polyketide Synthase Gene Cluster in Streptomyces aureofaciens CCM 3239

We cloned a new polyketide gene cluster, aur2, in Streptomyces aureofaciens CCM3239. Sequence analysis of the 9531-bp DNA fragment revealed 10 open reading frames, majority of which showed high similarity to the previously characterized type II polyketide synthase (PKS) genes. An unusual feature of...

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Bibliographic Details
Published in:DNA sequence 2004-06, Vol.15 (3), p.188-195
Main Authors: Novakova, Renata, Bistakova, Jana, Homerova, Dagmar, Rezuchova, Bronislava, Feckova, Lubica, Kormanec, Jan
Format: Article
Language:English
Subjects:
Actinomycetes
Antibiotics
AY033994
Base Sequence
Blotting, Southern
Cloning, Molecular
Differentiation
Electrophoresis, Agar Gel
Gene Components
Molecular Sequence Data
Multigene Family - genetics
Physical Chromosome Mapping
Polyketide
Polyketide Synthases - genetics
Polyketide Synthases - physiology
Sequence Analysis, DNA
Streptomyces aureofaciens
Streptomyces aureofaciens - genetics
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Summary:We cloned a new polyketide gene cluster, aur2, in Streptomyces aureofaciens CCM3239. Sequence analysis of the 9531-bp DNA fragment revealed 10 open reading frames, majority of which showed high similarity to the previously characterized type II polyketide synthase (PKS) genes. An unusual feature of the aur2 cluster is a disconnected organization of minimal PKS genes; ACP is located apart from the genes for ketosynthases KSα and KS . The aur2 gene cluster was disrupted in S. aureofaciens CCM3239 by a homologous recombination, replacing the four genes (aur2A, E, F, G) including ketosynthase KSα, with antibiotic resistance marker gene. The disruption did not affect growth and differentiation, and disrupted strain produced spores with wild-type grey-pink pigmentation. The biochromatographic analysis of the culture extracts from S. aureofaciens wild type and aur2-disrupted strains did not reveal any difference in the pattern of antibacterial compounds.
ISSN:1042-5179
1029-2365
DOI:10.1080/10425170410001710556