Clearing and embedding in polyester resin for demonstrating the nerve distribution pattern of skeletal muscles

The preservation of many stained gross specimens in solution creates some difficulties. It is convenient and effective to preserve material in polyester resin instead of glycerol. The aim of this study was to determine the usefulness of clearing and embedding using polyester resin. The samples consi...

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Bibliographic Details
Published in:Biotechnic & histochemistry 2003-06, Vol.78 (3-4), p.187-190
Main Authors: Peker, T, Gülekon, N, An l, A, Turgut, Hb, Liman, F
Format: Article
Language:English
Subjects:
embedding
Glycerol
innervation pattern
Muscle, Skeletal - anatomy & histology
Muscle, Skeletal - innervation
Nerve Tissue - anatomy & histology
Plastic Embedding - methods
polyester
Polyesters
Sihler's stain
skeletal muscle
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Summary:The preservation of many stained gross specimens in solution creates some difficulties. It is convenient and effective to preserve material in polyester resin instead of glycerol. The aim of this study was to determine the usefulness of clearing and embedding using polyester resin. The samples consisted of the nerve distribution patterns of skeletal muscles stained using Sihler's method. The muscles were cleared more successfully and the intramuscular nerve distributions were demonstrated better in polyester than in glycerol. The method presented here eliminates not only the storage and handling problems of specimens, but also problems such as pale stains and the molding of preparations. Furthermore, it is more convenient to examine and to photograph specimens cleared and embedded in polyester than those stored in glycerol.
ISSN:1052-0295
1473-7760
DOI:10.1080/10520790310001594026