Exercise training and oxidative stress in the elderly as measured by antipyrine hydroxylation products

Effects of 12 wk exercise training on oxidative stress were examined in elderly humans. We measured oxidative stress during a 45 min cycling test by using antipyrine hydroxylation products. Antipyrine breakdown is independent of blood flow to the liver, which is important during exercise. Furthermor...

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Bibliographic Details
Published in:Free radical research 2001-01, Vol.35 (4), p.435-443
Main Authors: Meijer, Erwin P., Coolen, Stefan A.J., Bast, Aalt, Westerterp, Klaas R.
Format: Article
Language:English
Subjects:
aging
Aging - physiology
Antipyrine
Antipyrine - blood
aromatic hydroxylation
Chromatography, High Pressure Liquid
Exercise - physiology
Female
Gas Chromatography-Mass Spectrometry
Humans
hydroxyl radicals
Hydroxylation
Male
Middle Aged
Oxidative Stress
Oxygen Consumption
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Summary:Effects of 12 wk exercise training on oxidative stress were examined in elderly humans. We measured oxidative stress during a 45 min cycling test by using antipyrine hydroxylation products. Antipyrine breakdown is independent of blood flow to the liver, which is important during exercise. Furthermore, antipyrine reacts quickly with hydroxyl radicals to form para- and ortho-hydroxyantipyrine. Ortho-hydroxyantipyrine is not formed in man through the mono-oxygenase pathway of cytochrome P450. Twenty subjects (9 women; 60 ± 3 y) participated in the training program. Thirteen subjects (5 women; 64 ± 7 y) served as inactive controls. Subjects trained, twice a week for 1h, at a fitness center. After 12 wk, maximal oxygen uptake (p < .005) and workload capacity (p < .001) were only significantly elevated in the training group. After 12 wk, both groups observed no change in the ratios of antipyrine hydroxylates, para- and ortho-hydroxy-antipyrine, to native antipyrine. Furthermore, no differences were observed within or between groups in the exercise-induced increase in the plasma level of thiobarbituric acid reactive species. In conclusion, 12-wk training had no effect on exercise-induced oxidative stress in elderly humans as measured by free radical reaction products of antipyrine. Despite the fact that training in elderly humans improves functional capacity, it appears not to compromise antioxidant defense mechanisms.
ISSN:1071-5762
1029-2470
DOI:10.1080/10715760100300951