Diabetes-induced Activation of Caspase-3 in Retina: Effect of Antioxidant Therapy

Apoptosis of retinal endothelial cells and pericytes is postulated to contribute to the development of retinopathy in diabetes. The goal of this study is to investigate diabetes-induced activation of retinal caspase-3, an apoptosis executer enzyme, in retina, and examine the effects of antioxidants...

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Bibliographic Details
Published in:Free radical research 2002, Vol.36 (9), p.993-999
Main Authors: Kowluru, Renu A., Koppolu, Prashant
Format: Article
Language:English
Subjects:
Acetylcysteine - administration & dosage
alpha-Tocopherol - administration & dosage
Animals
Antioxidants
Antioxidants - therapeutic use
Apoptosis
Ascorbic Acid - administration & dosage
beta Carotene - administration & dosage
Caspase
Caspase 3
Caspases - metabolism
Diabetes
Diabetes Mellitus, Experimental - enzymology
Diabetic Retinopathy - enzymology
Diabetic Retinopathy - pathology
Diabetic Retinopathy - prevention & control
Endothelium, Vascular - enzymology
Endothelium, Vascular - pathology
Enzyme Activation
Glucose - metabolism
In Vitro Techniques
Male
Oxidative Stress
Pericytes - enzymology
Pericytes - pathology
Poly(ADP-ribose) Polymerases - metabolism
Rats
Rats, Sprague-Dawley
Retina - enzymology
Retinopathy
Selenium - administration & dosage
Thiobarbituric Acid Reactive Substances - metabolism
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Summary:Apoptosis of retinal endothelial cells and pericytes is postulated to contribute to the development of retinopathy in diabetes. The goal of this study is to investigate diabetes-induced activation of retinal caspase-3, an apoptosis executer enzyme, in retina, and examine the effects of antioxidants on the activation. Caspase-3 activation was determined in the retina of alloxan diabetic rats (2-14 months duration) and in the isolated retinal capillary cells (endothelial cells and pericytes) by measuring cleavage of caspase-3 specific fluorescent substrate, and cleavage of caspase-3 holoenzyme and poly (ADP ribosyl) polymerase. Effect of antioxidants on the activation of caspase-3 was determined by feeding a group of diabetic rats diet supplemented with a comprehensive mixture of antioxidants, including Trolox, &#102 -tocopherol, N -acetyl cysteine, ascorbic acid, &#103 -carotene and selenium for 2-14 months, and also under in vitro conditions by incubating isolated retinal capillary cells with antioxidants with wide range of actions. Caspase-3 was activated in the rat retina at 14 months of diabetes ( P
ISSN:1071-5762
1029-2470
DOI:10.1080/1071576021000006572