Boron-Loaded Liposomes in the Treatment of Hepatic Metastases: Preliminary Investigation by Autoradiography Analysis

Boronophenylalanine (BPA)-loaded conventional and stabilized liposomes were prepared by the reversed phase evaporation method to treat liver metastases by boron neutron capture therapy. Conventional vesicles were composed of phosphatidylcholine and cholesterol, molar ratio 1:1. To obtain stealth lip...

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Bibliographic Details
Published in:Drug delivery 2000-04, Vol.7 (2), p.97-103
Main Author: Franca Pavanetto, Paola Perugini, Ida Genta, Claudio Minoia, Anna Ronchi, Ubaldo Prati, Laura Roveda, Rosanna Nano
Format: Article
Language:English
Subjects:
Animals
Antitumor Therapy Boron Neutron Capture Therapy Bnct Boronophenylalanine Liposomes
Autoradiography
Boron Compounds - administration & dosage
Boron Neutron Capture Therapy
Liposomes
Liver Neoplasms - radiotherapy
Liver Neoplasms - secondary
Phenylalanine - administration & dosage
Phenylalanine - analogs & derivatives
Polyethylene Glycols - administration & dosage
Radiation-Sensitizing Agents - administration & dosage
Rats
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Summary:Boronophenylalanine (BPA)-loaded conventional and stabilized liposomes were prepared by the reversed phase evaporation method to treat liver metastases by boron neutron capture therapy. Conventional vesicles were composed of phosphatidylcholine and cholesterol, molar ratio 1:1. To obtain stealth liposomes, GM or PEG were included in the lipidic bilayer at a concentration of 6.67 or 5 mol%, respectively. Large unilamellar vesicles were formulated encapsulating BPA in the liposome aqueous compartment as a complex with fructose; BPA free base also was embedded into the lipidic bilayer. In vivo experiments were carried out after intravenous injection of liposome suspensions in BD-IX strain rats in which liver metastases had been induced. Alpha particle spectroscopy associated with histological analysis was performed to visualize boron spatial distribution in liver. Simultaneously, tissue boron concentrations were determined using inductively coupled plasma-mass spectroscopy. Results showed that PEG-modified liposomes accumulated boron in therapeutic concentrations (> 30 mug boron/g tissue) in metastatic tissue. The PEG-liposomes could be further explored in enhancing boron delivery to tumor cells.
ISSN:0049-8254
1071-7544
1366-5928
1521-0464
DOI:10.1080/107175400266669